We narrowed to 1,801 results for: PORA

1. pMRXIP-GFP-PKD C1ab

   Plasmid

   #221755

   Purpose

   Stable expression of GFP-PKD C1ab in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   Prkd1(138-343) (Prkd1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 138-343

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr

   Plasmid

   #218766

   Purpose

   encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

   Insert

   EcTyrRS-VSMA*
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y37V, D167G, D182S, F183M, L186A, D265R

   Promoter

   tacI

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

   Plasmid

   #218767

   Purpose

   encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

   Insert

   EcTyrRS-pAAFRS-9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y37G, L71V, D182C, F183Y, L186C, D265R

   Promoter

   tacI

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-Nova1-GFP

   Plasmid

   #217033

   Purpose

   Overexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neurons

   Depositor

   Chaolin Zhang

   Article

   Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

   Insert

   Nova1 (Nova1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TDP-43 mRuby K84ONBK

   Plasmid

   #216226

   Purpose

   TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   Transactive response DNA binding protein of 43 kDa (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lysine 84 mutated to a TAG stop codon

   Promoter

   CMV Promoter

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-ST-P2A-pSC-CAAX

   Plasmid

   #207637

   Purpose

   A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   CMV

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Aga2p-LOV-Turbo1-myc_pCTCON2

   Plasmid

   #199673

   Purpose

   expresses pre-evolved version of LOV-Turbo on the yeast surface

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo1
   

   Use

   Tags

   Aga2p and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. iEscSnFR

   Plasmid

   #182807

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

   Insert

   iEscSnFR
   

   Use

   Tags

   6xHis, HA and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. iFluoxSnFR

   Plasmid

   #182808

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

   Insert

   iFluoxSnFR
   

   Use

   Tags

   6xHis, HA and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174881

    Purpose

    Targeting vector for generating Flnc STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Flnc
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174885

    Purpose

    Targeting vector for generating Usp5 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Usp5
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174886

    Purpose

    Targeting vector for generating Wnk1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Wnk1
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-Hygro-EF1A-KR-Linker-eGFP

    Plasmid

    #176066

    Purpose

    EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    KR
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b-ecDHFR-D27S/Y100F

    Plasmid

    #110827

    Purpose

    Bacterial expression of E.coli DHFR D27S/Y100F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D27 mutated to S27, Y100 mutated to F100

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

    Plasmid

    #107739

    Purpose

    mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein(C1A)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    change the first cysteine of gp41-1 N-intein to a…

    Promoter

    C. elegans myo-2 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAcBac1-Pyl-TAG

    Plasmid

    #228784

    Purpose

    Pyl aaRS/tRNA system for mammalian TAG suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    PylRS
    PytR
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Mfn2-YFP

    Plasmid

    #28010

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

    Insert

    mitofusin 2 (MFN2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    silent S508S

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLM-mCerulean-cMyc

    Plasmid

    #23244

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLM-mCherry-Klf4

    Plasmid

    #23243

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EFS-DIO-GRAB_OT1.0

    Plasmid

    #185387

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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