We narrowed to 3,021 results for: PHI-1

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SUPERBLADE5

Plasmid

#134913

Purpose

Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Promoter

U6

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NOD1-EGFP

Plasmid

#131206

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Insert

NOD1 (NOD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG1856_A

Plasmid

#145905

Purpose

Insect Expression of CG1856-3UTR

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jan . 15(1):21-32.

Insert

CG1856-3UTR (ttk Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pp-CBBC-Fluc

Plasmid

#232920

Purpose

Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.

Depositor

Philippe Mangeot

Article

Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP

Plasmid

#58472

Purpose

Expresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Utr230 (UTRN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr261-EGFP

Plasmid

#58471

Purpose

Expresses a cytoplasmic actin filament reporter, the tandem CH domains (a.a. 1-261) from human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Utr261 (UTRN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Agam_557

Plasmid

#176667

Purpose

Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Agam_557

Plasmid

#176653

Purpose

Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48

Plasmid

#17605

Purpose

Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-NOD2-EGFP

Plasmid

#131207

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Insert

NOD2 (NOD2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAR100

Plasmid

#180377

Purpose

pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).

Depositor

Alec Redwood

Article

Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:

Inserts

NFAT-Luciferase
hCD8A

Expression

Bacterial and Mammalian

Promoter

hEF1-HTLV and minimal promoter and an NFAT respon…

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-E2F1-PIP3A

Plasmid

#37142

Depositor

Robert Duronio

Article

Shibutani et al Dev Cell. 2008 Dec;15(6):890-900.

Insert

E2F1-P!P3A (E2f1 Fly)

Use

Gateway Cloning

Mutation

I153, Y156, Y157 to Alanine

Available Since

July 9, 2012

Availability

Academic Institutions and Nonprofits only

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pFOSGE_CUS1F03

Plasmid

#71071

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Su(var)205 (Su(var)205 Fly)

Use

Gateway Cloning

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
pRSFDuetG-DmDCP1_128-372 (MCSI)_E

Plasmid

#146174

Purpose

Bacterial Expression of DmDcp1_128-372

Depositor

Elisa Izaurralde

Insert

DmDcp1_128-372 (Dcp1 Fly)

Expression

Bacterial

Mutation

3 amino acid del (PQQ 318-320)

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG3048_A

Plasmid

#145856

Purpose

Insect Expression of CG3048-3UTR

Depositor

Elisa Izaurralde

Insert

CG3048-3UTR (Traf4 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRL-TK 4x wt

Plasmid

#11313

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pZA1

Plasmid

#158479

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)

Plasmid

#225437

Purpose

Transcriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

CBS-att-OCS-BeYDVRep/RepA-tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)

Plasmid

#225431

Purpose

OCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-att-OCSterm-att-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pZA3

Plasmid

#158481

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, STOP codon

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZA2

Plasmid

#158480

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

D489V, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA11

Plasmid

#158489

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E no STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA9

Plasmid

#158487

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

no STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA4

Plasmid

#158482

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, D489V, STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA10

Plasmid

#158488

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

D489V, no STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GAG-CRErec

Plasmid

#119971

Purpose

Expresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE protein

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GAG-nlsCRErec

Tags

nls

Expression

Mammalian

Promoter

hCMV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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actin CP alpha 2 beta 2

Plasmid

#13452

Depositor

John Cooper

Article

Bhattacharya et al J Biol Chem. 2006 Oct 13. 281(41):31021-

Insert

capping protein alpha 2 beta 2 (mouse) (Capzb, Capza2 Mouse)

Expression

Bacterial

Mutation

none

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
BICstim-Gag-dCAS9-VPR

Plasmid

#120922

Purpose

encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GAG (FMLV)-dCAS9-VPR

Tags

none

Expression

Mammalian

Promoter

hCMV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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