We narrowed to 1,801 results for: αGFP

Showing: 1121 - 1140 of 1801 results

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pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

Plasmid

#188773

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

Plasmid

#213117

Purpose

Expression of a faster-running MinE mutant in mammalian cells

Depositor

Scott Coyle

Publication

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE(L4E)-EGFP (minE Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Mutation

L4E

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1079 - pAAV TH gRNA A EF1a EGFP

Plasmid

#113159

Purpose

An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Publication

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

Plasmid

#209080

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

Depositor

Vikram Khurana

Publication

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

A53T

Promoter

TRE4G

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGG-cTP-TurboID-GFP

Plasmid

#209403

Purpose

Transiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in planta

Depositor

Rosa Lozano-Durán

Publication

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

PM-3XHA-TurbOID

Tags

GFP

Expression

Plant

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-Cx43-GFP

Plasmid

#176860

Purpose

Expresses Cx43 fused with GFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Publication

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Cx43-eGFP (Gja1 Mouse)

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4 mito roGFP2-Orp1

Plasmid

#65001

Purpose

Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

Depositor

Tobias Dick

Publication

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLL5.0-MRLC-DD-GFP

Plasmid

#187260

Purpose

Lentiviral expression of myosin regulatory light chain-DD mutant

Depositor

Alpha Yap

Publication

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Mutation

T18D, S19D

Promoter

5' LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLL5.0-MRLC-AA-GFP

Plasmid

#187259

Purpose

Lentiviral expression of myosin regulatory light chain-AA mutant

Depositor

Alpha Yap

Publication

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Mutation

T18A,S19A

Promoter

5' LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-WT-GFP

Plasmid

#187258

Purpose

Lentiviral expression of myosin regulatory light chain-WT

Depositor

Alpha Yap

Publication

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Promoter

5' LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD EGFP

Plasmid

#187267

Purpose

Lentiviral expression of shRNA targeting Cortactin

Depositor

Alpha Yap

Publication

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

shRNA for Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP2AKAT7(E508Q)-W

Plasmid

#159293

Purpose

Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

Depositor

Kosuke Yusa

Publication

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

KAT7 E508Q

Use

Lentiviral

Expression

Mammalian

Mutation

CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

Promoter

human EF1a promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 EGFP-guide1

Plasmid

#118166

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the EGFP CDS

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta6

Plasmid

#101294

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Publication

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

375-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97311

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

Plasmid

#48253

Purpose

LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

CRY2PHR-NLS-VP64

Use

AAV; Tale

Tags

2A_GFP

Expression

Mammalian

Promoter

EF1-alpha

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Nup54-mEGFP494(0)

Plasmid

#163426

Purpose

Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of the mEGFP

Depositor

Sanford Simon

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nup54 (NUP54 Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C-SLC35B2

Plasmid

#155037

Purpose

Transient expression of EGFP-SLC35B2

Depositor

Yihong Ye

Publication

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SLC35B2 (SLC35B2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-neo:Beta2meGFP

Plasmid

#45096

Depositor

Henry Lester

Publication

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nAChR beta2 (Chrnb2 Mouse)

Expression

Mammalian

Mutation

There are strain differences between C57BJ/6 and …

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV mRuby3-Galectin-3

Plasmid

#215375

Purpose

Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mRuby3 tag.

Depositor

Franz-Ulrich Hartl

Publication

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LGALS3 (LGALS3 Human)

Expression

Mammalian

Available since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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