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pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1

Plasmid

#10880

Depositor

Patrick Aebischer

Publication

Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

Insert

H1-shSOD1 (SOD1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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mVenus-PA-PKI

Plasmid

#65456

Purpose

photoactivatable PKA inhibition

Depositor

Klaus Hahn

Publication

Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

Insert

PKI (PKIA, Pkia Synthetic)

Expression

Mammalian

Promoter

CMV

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCAG-HA-rat Nrxn3beta AS4(-)

Plasmid

#58269

Purpose

Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Publication

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn3beta AS4(-) (Nrxn3 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EasyFusion T2A-H2B-mCherry

Plasmid

#113089

Purpose

General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-mCherry

Use

Synthetic Biology

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro Xlone-eGFP

Plasmid

#136936

Purpose

donor plasmid for inducible transgene expression in human cells

Depositor

Xiaoping Bao

Publication

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

all-in-one tet-on system

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GS

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKMD111e - intein-Cas9(S219-G521R)

Plasmid

#64192

Purpose

Expresses intein-Cas9(S219-G521R) in mammalian cells

Depositor

David Liu

Publication

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FUW-RLuc-T2A-PuroR

Plasmid

#102320

Purpose

expression of r-luciferase

Depositor

Rudolf Jaenisch

Publication

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Renilla luciferase

Use

Lentiviral

Promoter

Ubc

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_V5-APEX2-HMOX1

Plasmid

#85824

Purpose

Expressed N-term tagged APEX2 on HMOX1 in mammalian cell

Depositor

Hyun-Woo Rhee

Publication

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-HMOX1 (HMOX1 Synthetic, Human)

Tags

APEX2 and V5

Expression

Mammalian

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Publication

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Promoter

Cbh

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

Plasmid

#63168

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-V5"

Tags

N-myristoylation

Expression

Insect

Promoter

GAL4-responsive UAS hsp70

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCFJ906

Plasmid

#44472

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, [4-3] Gateway

Use

Gateway Cloning

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-HSD17B11-WT-FLAGC

Plasmid

#161903

Purpose

Expresses human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Publication

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)

Tags

FLAG-GFP

Expression

Mammalian

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-p300-DY1399

Plasmid

#89095

Purpose

mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect

Depositor

Elizabeth Wilson

Publication

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300-D1399Y mutant (EP300 Human)

Tags

HA

Expression

Mammalian

Mutation

p300-DY1399

Promoter

SV40

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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PBS Actb-2A-TIR1-GFP

Plasmid

#113832

Purpose

targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

osTIR1-GFP (NEWENTRY Synthetic)

Use

Synthetic Biology

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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EasyFusion eGFP

Plasmid

#112848

Purpose

General donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

EGFP

Use

Unspecified; General donor

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEYFP-hDF

Plasmid

#72584

Purpose

human dysferlin full length tagged with YFP

Depositor

Renzhi Han

Publication

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-miRFP703

Plasmid

#113097

Purpose

General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-miRFP703

Use

Synthetic Biology

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pdCas9-DNMT3A-PuroR_hNT

Plasmid

#71830

Purpose

Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

Depositor

Vlatka Zoldos

Publication

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

Non-targeting sgRNA human (DNMT3A S. pyogenes, Synthetic, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

U6

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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tubP-FRT>GAL80-6-FRT>

Plasmid

#63173

Purpose

ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"Flp-Out" GAL80

Expression

Insect

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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