Addgene

We narrowed to 150,861 results for: DES

Showing: 1121 - 1140 of 150861 results

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pDEST17-His10-ccdB

Plasmid

#166145

Purpose

Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Type

Empty backbone

Use

Tags

His10

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pR26 GFP Dest

Plasmid

#74283

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Tags

Expression

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST17-hTRPT1-iso1

Plasmid

#184239

Purpose

For purification of N-terminally His-tagged TRPT1 from bacteria

Depositor

Karla Feijs

Article

Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

Insert

TRPT1 (TRPT1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCFJ212 - pDESTR4-R3(cxTi10816, IV)

Plasmid

#34864

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

cxTi10816 targeting

Use

Worm targeting

Tags

Expression

Mutation

Promoter

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST/LIfeAct-mCherry-N1

Plasmid

#40908

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

LifeAct

Use

Tags

mCherry

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-DogCatcher

Plasmid

#171929

Purpose

Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

Cys-DogCatcher

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-N

Plasmid

#118366

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-C

Plasmid

#118367

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-G2019S

Plasmid

#29401

Depositor

Mark Cookson

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Gly2019Ser

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL8

Plasmid

#149519

Purpose

Express APEX2-Galectin-8 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS8 (LGALS8 Human)

Use

Tags

APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST53-LRRK2-G2019S

Plasmid

#25045

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Glycine 2019 mutated to Serine

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL3

Plasmid

#149518

Purpose

Express APEX2-Galectin-3 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS3 (LGALS3 Human)

Use

Tags

APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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JC201: pMVP/Ad/5-35-DEST

Plasmid

#121847

Purpose

pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL3-GFP

Plasmid

#67397

Purpose

Mammalian expression of hArl3 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pLentiCMVNeoDEST(705-1)-hAXL

Plasmid

#124796

Purpose

Expresses AXL in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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1136- Desmoplakin-GFP

Plasmid

#32227

Depositor

Kathleen Green

Article

Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

Insert

Desmoplakin (DSP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut4

Plasmid

#18087

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut4 (SLC2A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-EGFP

Plasmid

#122842

Purpose

N-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/V5-DEST-GFP

Plasmid

#40125

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST/hCx43-EGFP-N1

Plasmid

#40907

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 (GJA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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