We narrowed to 3,367 results for: aaas

1. pCHCHD3-N2.1.2-gDNA

   Plasmid

   #175858

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   CHCHD3-Nickase2
   

   Use

   CRISPR

   Available Since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC102-MBP-eIF4G-213

   Plasmid

   #219974

   Purpose

   eIF4G-213 protein purification

   Depositor

   Simon Alberti

   Insert

   eIF4G-213 (TIF4631 Budding Yeast)
   

   Tags

   6xHis-MBP

   Expression

   Insect

   Mutation

   213VKLR216 to 213AAAA216

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfb13209

   Plasmid

   #219884

   Purpose

   The base plasmid of TUNEYALI for TF19

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF19 (YALI1_A20855g) and homologous arm matching TF19
   

   Expression

   Yeast

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfb13229

   Plasmid

   #219904

   Purpose

   The base plasmid of TUNEYALI for TF39

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF39 (YALI1_B16808g) and homologous arm matching TF39
   

   Expression

   Yeast

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfb13212

   Plasmid

   #219887

   Purpose

   The base plasmid of TUNEYALI for TF22

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF22 (YALI1_C25288g) and homologous arm matching TF22
   

   Expression

   Yeast

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPHTF2.1.0-gDNA

   Plasmid

   #132465

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   PHTF2 (PHTF2 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458_ZNF3_2

   Plasmid

   #72364

   Purpose

   Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFP

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   ZNF3 (ZNF3 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. ipUSEPR-sg-Hs-PHGDH-int1

   Plasmid

   #188681

   Purpose

   control sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH intron control (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRARB.1.0-gDNA

   Plasmid

   #132471

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   RARB (RARB Human)
   

   Use

   CRISPR

   Available Since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJR255

    Plasmid

    #78549

    Purpose

    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.

    Depositor

    John Rossi

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    U6, CMV

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK217

    Plasmid

    #72471

    Purpose

    Produces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)

    Depositor

    T. J. Kappock

    Insert

    citrate synthase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-TRC.mKO2_shHuR CDS

    Plasmid

    #110426

    Purpose

    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAVL1 HuR
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA Pol III)

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNSU299_SEO1

    Plasmid

    #166076

    Purpose

    Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    SEO1 (SEO1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    Tet-inducible

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML107-VHT1

    Plasmid

    #232898

    Purpose

    Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    VHT1 gRNA (VHT1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    snR52

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. ipUSEPR-sg-Hs-CDK1

    Plasmid

    #188684

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgCDK1 (CDK1 Human)
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNSU299_RRT8

    Plasmid

    #166080

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Promoter of RRT8 (RRT8 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    Tet-inducible

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJR288

    Plasmid

    #78550

    Purpose

    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.

    Depositor

    John Rossi

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pML104-HygMx4-URA3-3

    Plasmid

    #232886

    Purpose

    Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    URA3 gRNA (URA3 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    snR52

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pML104-HIS3-3

    Plasmid

    #232882

    Purpose

    Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    HIS3 gRNA (HIS3 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    snR52

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pML107-ZIM17

    Plasmid

    #232901

    Purpose

    Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    ZIM17 gRNA (ZIM17 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    snR52

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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