We narrowed to 1,246 results for: calcium

1. pRSET a sfMatryoshCaMP6s-T78H

   Plasmid

   #100021

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfMatryoshCaMP6s
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP and T78H m…

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-GCaMP3-44

   Plasmid

   #91780

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP3-44
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP Cav2.2 W391A pRK5

   Plasmid

   #206084

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

   Depositor

   Annette Dolphin

   Article

   Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   W391A

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cav2.2 HA W391A pcDNA3

   Plasmid

   #206071

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

   Depositor

   Annette Dolphin

   Article

   Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W391A

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CMV-GCaMP6m

   Plasmid

   #40754

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6m
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

   Plasmid

   #194681

   Purpose

   CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_01-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

   Plasmid

   #194682

   Purpose

   CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_04-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGP-CMV-GCaMP6f

   Plasmid

   #40755

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6f
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGP-CMV-GCaMP6s

   Plasmid

   #40753

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6s
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218863

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGolt-mCherry / pGolt3-mCherry

    Plasmid

    #73297

    Purpose

    Visualization of Dendritic Golgi Satellite

    Depositor

    Michael Kreutz

    Article

    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

    Inserts

    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    

    Use

    Tags

    ER export and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2-elavl3-H2B-GCaMP6s

    Plasmid

    #59530

    Purpose

    Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    H2B
    GCaMP6s
    

    Use

    Transposable element

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194693

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-puro_CMV_NES-Caprola_on-mEGFP

    Plasmid

    #194694

    Purpose

    CMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194695

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

    Plasmid

    #194683

    Purpose

    CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1a-GCaMP6s-WPRE-pGHpA

    Plasmid

    #67526

    Purpose

    "Fluorescent reporter for calcium imaging"

    Depositor

    Botond Roska

    Article

    Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    6xHis, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3_erGAP3

    Plasmid

    #78118

    Purpose

    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

    Depositor

    Javier García-Sancho, Teresa Alonso

    Article

    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

    Insert

    erGAP3
    

    Use

    Tags

    KDEL and calreticulin signal peptide

    Expression

    Mammalian

    Mutation

    S175G, D180Y, I167V and L15Q in GFP moiety and D…

    Promoter

    CMV

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-CMV-GCaMP6-150

    Plasmid

    #91777

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP6-150
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CaV1.3e[8a,11,31b,Δ32,42a]

    Plasmid

    #49333

    Purpose

    Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    CMV

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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