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pcDNA3-CMV-eBRIC

Plasmid

#241800

Purpose

Bioluminescence indicator for Ca2+ imaging in mammalian cells

Depositor

Huiwang Ai

Article

Tian et al ACS Sens. 2025 Jul 29. doi: 10.1021/acss

Insert

eBRIC

Expression

Bacterial

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pNK1508

Plasmid

#219750

Purpose

pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - npgA - tAOX

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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SP_H6_HaloK73T_KDEL_pCMV1

Plasmid

#183686

Purpose

Mammalian expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)

Expression

Mammalian

Mutation

K73T in Halotag

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET30_Halotag_K73T_v2

Plasmid

#183692

Purpose

Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)

Expression

Bacterial

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET_scFv_QLuc

Plasmid

#183261

Purpose

Express scFv_QLuc in bacteria cells

Depositor

Huiwang Ai

Article

Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

Insert

scFv_QLuc

Tags

Histag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSSa16

Plasmid

#177372

Purpose

Expresses red-shifted luciferase under ENO1 promoter in Candida glabrata

Depositor

Dominique Sanglard

Article

Schrevens et al J Fungi (Basel). 2021 Oct 9;7(10). pii:

Insert

CgLUCopt-mut2

Promoter

ENO1

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSSa23

Plasmid

#177373

Purpose

Expresses red-shifted luciferase under TEF1 promoter in Candida glabrata

Depositor

Dominique Sanglard

Article

Schrevens et al J Fungi (Basel). 2021 Oct 9;7(10). pii:

Insert

CgLUCopt-mut2

Promoter

TEF1

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pX029

Plasmid

#167152

Purpose

MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnH3H-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pX020

Plasmid

#167146

Purpose

MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

fungal caffeoylpiruvate hydrolase, nnCPH

Use

Synthetic Biology

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCO706

Plasmid

#139743

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaC

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

hpaB

Use

Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCO707

Plasmid

#139744

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaC

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

hpaC

Use

Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-luxKate

Plasmid

#229946

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKate

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pNK5867

Plasmid

#219751

Purpose

pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - mcitHispS - tAOX

Expression

Yeast

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMGU

Plasmid

#139933

Purpose

M. magneticum/E. coli shuttle vector (pMGT and pUC)

Depositor

Christopher Voigt

Article

Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23101

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_HaloK73T_cyto

Plasmid

#183687

Purpose

Mammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

Cyto Halo(K73T)

Expression

Mammalian

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCO708

Plasmid

#139745

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaC

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

TAL

Use

Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BRIPO

Plasmid

#215542

Purpose

BRIPO (Bioluminescent Red Indicator for Potassium)

Depositor

Huiwang Ai

Article

Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

Insert

BRIPO

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-BRIC

Plasmid

#172343

Purpose

Bioluminescent Red Indicator for Calcium (bacterial expression)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BRIC

Expression

Bacterial

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDS1902

Plasmid

#167278

Purpose

Expression plasmid for Candida albicans containing a red-shifted firefly luciferase

Depositor

Dominique Sanglard

Article

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Insert

red shifted luciferase

Promoter

ACT1

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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P442

Plasmid

#139728

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz, promoter is ABA responsive

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_D_pAtRAB18:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

AtRAB18

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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