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pKHH030_sgCtrl#1

Plasmid

#174142

Purpose

Lentiviral vector expressing a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_AMBRA1#1

Plasmid

#174152

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-TOMM20-mEGFP

Plasmid

#115923

Purpose

donor to insert EGFP at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-mEGFP

Use

Donor template

Tags

EGFP

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-ACTB-mEGFP

Plasmid

#115917

Purpose

donor to insert EGFP at the ACTB locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

ACTB-mEGFP

Use

Donor template

Tags

EGFP

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJFRC-20XUAS-IVS-PhiC31

Plasmid

#133565

Purpose

Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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2xsspB-mSc-sspB

Plasmid

#114423

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Ubiquitin (1-76; T66V, L67N)

Plasmid

#110755

Purpose

Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

Depositor

David Komander

Article

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Expression

Bacterial

Mutation

T66V, L67N

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Reb_140 aka PAS625

Plasmid

#71230

Purpose

Reb_1 with RebA(S88P)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(S88P)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pGL3-DSCP-Gateway

Plasmid

#71507

Purpose

flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLPC-CHES1L

Plasmid

#53743

Purpose

long isoform of human CHES1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pFAB3689

Plasmid

#47834

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 3 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 WT

Plasmid

#32392

Depositor

Julien Sage

Article

Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pEN_hU6miR-Luc-A

Plasmid

#25756

Purpose

Entry vector with human U6 promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Gateway Cloning

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pPB.DEST-ITGB1(WT)-mRuby2

Plasmid

#215448

Purpose

Piggybac expression vector with integrin beta1 tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Transposon-based stable expression

Tags

mRuby2

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA

Plasmid

#193728

Purpose

Cre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell types

Depositor

Colin Akerman

Article

Calin et al iScience. 2023 Mar 9;26(4):106363. doi:

Insert

ClopHensorN

Use

AAV

Tags

Strep-II (N terminal on insert)

Expression

Mammalian

Promoter

EF1a

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag DYRK2

Plasmid

#20479

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

DYRK2 (DYRK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 D500-630

Plasmid

#19841

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D500-630 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 500-630

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pCMV_aActTS_indicator

Plasmid

#252385

Purpose

Non-FRET aActinin tension indicator. cpEGFP with elastic linker is inserted at the hinge with C-term mCherry.

Depositor

Maretoshi Hirai

Article

Fujiwara et al Commun Biol. 2026 Feb 19. doi: 10.1038/s

Insert

aActinin tension indicator

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pLX208-cytoFLARE1.0-TF

Plasmid

#234513

Purpose

Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG

Use

Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pIVT-mGL

Plasmid

#232768

Purpose

IVT template for making modRNA encoding mGreenLantern

Depositor

Kate Galloway

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mGreenLantern

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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