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pTC-ApoE-Tet

Plasmid

#85578

Purpose

Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver

Depositor

Ursula Ehmer

Article

Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

Insert

CreER

Use

Cre/Lox

Expression

Mammalian

Promoter

ApoE.HCR.hAAT

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB206

Plasmid

#68597

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO DEST (775-1)

Plasmid

#17432

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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PB-TET

Plasmid

#20909

Purpose

piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Gateway Destination Vector

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120392

Purpose

pAAV plasmid with BspQI sites for insertion of target specific shRNAs in frame of the murine U6 promoter and co expressing mCherry.3XFLAG under the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Type

Empty backbone

Use

AAV

Tags

mCherry.3XFLAG

Promoter

U6

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSF280

Plasmid

#167675

Purpose

Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-tag-C

Plasmid

#108077

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pC018 - LshCas13a from Leptotrichia shahii

Plasmid

#91908

Purpose

Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LshCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRU324

Plasmid

#167691

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDest_pK7WG2_pRPS5A_CTP OleGFP

Plasmid

#171723

Purpose

ptpTALECD vector assembly, Destination vector;

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2

Expression

Plant

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pC020 - LseCas13a from Listeria seeligeri

Plasmid

#91910

Purpose

Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LseCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pUBQ10-SNAP-GW

Plasmid

#209350

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AEB-Theo45

Plasmid

#63850

Purpose

This pFTV1 vector contains a theophylline binding riboswitch, controlling the translation initaition rate of mRFP1 gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

Theo45 theophylline riboswitch

Use

Synthetic Biology

Promoter

J23100

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Sth1Cas9n cytosine base editor

Plasmid

#194209

Purpose

Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

Sth1Cas9-D9A nickase

Use

CRISPR

Expression

Bacterial

Mutation

Changed Aspartate 9 to Alanine.

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCBC-MT3T4

Plasmid

#50595

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T3, gRNA scaffold, U6-26t plus, OsU3p, T4

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCA24N_6xHis-TEV-rpsA

Plasmid

#83585

Purpose

Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

Depositor

Nils Walter

Article

Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

Insert

rpsA (rpsA Escherichia coli)

Tags

His tag

Expression

Bacterial

Promoter

p-T5 Lac

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTRV2-GG

Plasmid

#105349

Purpose

virus-induced gene silencing

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

TRV-RNA2(5')

Use

In planta gene silencing; viral

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR1-SV40-puro

Plasmid

#240535

Purpose

Lentiviral eBAR1 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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