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pLentiCRISPRv2_mSTING_gRNA_1

Plasmid

#196625

Purpose

Knock-out STING in murine cells, gRNA 1.

Depositor

Nicolas Manel

Publication

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING gRNA 1 (Sting1 Mouse)

Use

CRISPR and Lentiviral

Mutation

WT

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-BAF

Plasmid

#172447

Purpose

Expression of nuclear protein BAF with an N-terminal mCherry tag

Depositor

Stephen Royle

Publication

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

BAF

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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HLA_A0101

Plasmid

#165009

Purpose

Expression of HLA-A*01:01 cDNA

Depositor

Matthew Meyerson

Publication

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-A*01:01 (HLA-A Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Publication

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Expression

Mammalian

Promoter

U6

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pET_RGG-RGG

Plasmid

#124941

Purpose

Expresion of RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-mPCSK9

Plasmid

#122678

Purpose

Expresses mouse PCSK9 in mammalian cells

Depositor

Liming Pei

Publication

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 9 (Pcsk9) (Pcsk9 )

Expression

Mammalian

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HA-TRIM28deltaRING

Plasmid

#124952

Purpose

Mammalian expression of Human TRIM28 with deleted RING domain

Depositor

Michelle Barton

Publication

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of RING domain

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SP6-hCas9-Ce-mRNA

Plasmid

#47911

Purpose

In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

Depositor

Paul Sternberg

Publication

Chiu et al Genetics. 2013 Aug 26.

Insert

hCas9 with C. elegans 5' and 3' UTRs

Use

CRISPR

Promoter

SP6

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTK82_GFP-4.1G

Plasmid

#46353

Depositor

Iain Cheeseman

Publication

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G (EPB41L2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA4-Myc-HisB-Sirt3

Plasmid

#24918

Depositor

Toren Finkel

Publication

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
TRE.IkBSR-Flag Pgk.Cre

Plasmid

#22504

Depositor

Tyler Jacks

Publication

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

IkBSR

Use

Cre/Lox and Lentiviral

Tags

Flag

Expression

Mammalian

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ub[9]-mRFP[1]

Plasmid

#20249

Depositor

Nico Dantuma

Publication

Salomons et al Mol Cell Biol. 2009 Apr;29(7):1774-85. d

Insert

ubiquitin C (UBC Human)

Tags

mRFP

Expression

Mammalian

Mutation

changed Stop codon 686 to Glycine

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR4 TNRC6A

Plasmid

#20021

Depositor

Thomas Tuschl

Publication

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6A (TNRC6A Human)

Use

Gateway Cloning

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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SV40 basal promoter-LacZ

Plasmid

#18816

Depositor

Connie Cepko

Publication

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-LacZ

Expression

Mammalian

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MARCKS(1-40)-paGFP

Plasmid

#18695

Depositor

Karel Svoboda

Publication

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

MARCKS(1-40) (MARCKS Human)

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti:FlipGFP-T2A-mCherry

Plasmid

#223198

Purpose

Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

Depositor

Yeh-Hsing Lao

Publication

Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

Insert

FlipGFP-T2A-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP/golgin-160

Plasmid

#180630

Purpose

Expression of WT golgin-160

Depositor

Carolyn Machamer

Publication

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Insert

golgin-160 (GOLGA3 Human)

Tags

EGFP

Expression

Mammalian

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA221pmCDA1-T7RNAP

Plasmid

#167978

Purpose

Expresses fusion protein pmCDA1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Publication

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

pmCDA1-T7RNAP (T7p07 Synthetic)

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaPB

Plasmid

#124959

Purpose

Mammalian expression of Human TRIM28 with deleted PHD and BROMO domain

Depositor

Michelle Barton

Publication

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of PHD and Bromo Domain

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Halo-Cyto

Plasmid

#124314

Purpose

Expresses cytosolic specific HaloTag protein

Depositor

Jin Wang

Publication

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

Cytoplasm specific seq

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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