We narrowed to 23,305 results for: crispr

1. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

   Plasmid

   #113155

   Purpose

   An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat TH
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   mU6 and hU6

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

   Plasmid

   #113157

   Purpose

   An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat MANF
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   mU6 and hU6

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7 RNA POL

   Plasmid

   #138352

   Purpose

   Expressing T7 RNA Polymerase in mammalian cells

   Depositor

   Baisong Lu

   Insert

   T7 RNA POL
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLinker-2xTy-HXGPRT-LoxP

   Plasmid

   #86664

   Purpose

   CRISPR tagging with 2xTy in Toxoplamsa gondii

   Depositor

   David Sibley

   Insert

   Linker-2xTy-HXGPRT 3'UTR
   

   Use

   Expression in toxoplasma gondii

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROP-seq-opti-dsRed

   Plasmid

   #201999

   Purpose

   Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.

   Depositor

   Daniel Geschwind

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   dsRed

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRII-BbsI-sgRNAscaffold

   Plasmid

   #159352

   Purpose

   Expression vector for a customizable guide RNA

   Depositor

   Pim Pijnappel

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR6K-GFP-RFP

   Plasmid

   #199652

   Purpose

   R6K backbone with Golden Gate (GG) cloning sites

   Depositor

   IIya Finkelstein

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   J23119 promoter

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AgU6.695_3xP3-DsRed1-SV40 (Single gRNA)

   Plasmid

   #243009

   Purpose

   For cloning a single gRNA after BspQI digestion under An. gambiae U6.695 promoter.

   Depositor

   George Christophides

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Insect

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKS1603

   Plasmid

   #239527

   Purpose

   Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

   Depositor

   John Dueber

   Insert

   2xCrk_SH3-mTurquoise2-CpPT1(52-408)
   

   Expression

   Yeast

   Available Since

   July 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKS1616

    Plasmid

    #239547

    Purpose

    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS1615

    Plasmid

    #239546

    Purpose

    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKS1613

    Plasmid

    #239531

    Purpose

    Express CpPT1 membrane protein cargo fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    mTurquoise2-CpPT1(52-408)-SKL
    

    Expression

    Yeast

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1203R-CMV-3'dmDR-EGFP-bGHpolyA

    Plasmid

    #220962

    Purpose

    EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 3' end

    Depositor

    Omar Akbari

    Insert

    eGFP
    

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1203C-CMV-5’+3'DR-EGFP-bGHpolyA

    Plasmid

    #221454

    Purpose

    EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end and 3' end

    Depositor

    Omar Akbari

    Insert

    eGFP
    

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-Hygro

    Plasmid

    #127763

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

    Plasmid

    #191391

    Purpose

    Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(30-120)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mScarlet-I

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBSedit Sham

    Plasmid

    #223200

    Purpose

    Group B Streptococcus Efficient Genome Editing with Modular crRNA

    Depositor

    Thomas Hooven

    Insert

    N/A
    

    Use

    CRISPR

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TLCV2-LoxP

    Plasmid

    #127098

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system.

    Depositor

    Adam Karpf

    Insert

    Cas9-2A-eGFP
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_221

    Plasmid

    #169142

    Purpose

    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

    Depositor

    John Doench, David Root

    Insert

    EGFP , EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. XLone-PE2

    Plasmid

    #136463

    Purpose

    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Insert

    PE2
    

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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