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pKOdeltagalK (Kan)

Plasmid

#110089

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMGAHisTev5B

Plasmid

#37221

Depositor

Jon Lorsch

Publication

Acker et al Methods Enzymol. 2007;430:111-45.

Insert

eIF5B

Tags

His

Mutation

N-term truncation (contains aa 396-1002)

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only
pTYB2-eIF5

Plasmid

#37219

Depositor

Jon Lorsch

Publication

Acker et al Methods Enzymol. 2007;430:111-45.

Insert

eIF5

Expression

Bacterial

Promoter

T7

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

Plasmid

#191627

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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NUDT16L1

Plasmid

#155795

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

NUDT16L1 (NUDT16L1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKB224

Plasmid

#170014

Purpose

Encodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Publication

Brink et al bioRxiv

Inserts

mNeonGreen
NC

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 SARS-CoV-2 S-frag4_nostop

Plasmid

#153176

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag4

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag3_nostop

Plasmid

#153175

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag3

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221 SARS-CoV-2 S-frag1_nostop

Plasmid

#153173

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag1

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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myr-palm_2xmEYFP

Plasmid

#127811

Purpose

Homo-dimer mEYFP-mEYFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Publication

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

myristoylated-palmitoylated-mEYFP-mEYFP

Expression

Mammalian

Available since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-3xflagGREB1b

Plasmid

#112222

Purpose

mammalian expression of flag tagged GREB1b

Depositor

Craig Burd

Publication

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1b (GREB1 Human)

Tags

3x flag

Expression

Mammalian

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H08

Plasmid

#70996

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Nap1 (Nap1 Fly)

Use

Gateway Cloning

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pSG5-mERRbeta-short

Plasmid

#52188

Purpose

Expresses mouse ERRbeta short form splice variant in mammalian cells

Depositor

Rebecca Riggins

Publication

Heckler et al Cell Cycle. 2014 Dec 12:0.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Estrogen-related receptor beta (Esrrb Mouse)

Expression

Mammalian

Promoter

SV40

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pX28

Plasmid

#46850

Purpose

TOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiency

Depositor

Monica Hollstein

Publication

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB, RFP and PGK-puro

Expression

Mammalian

Promoter

PGK

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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NHA-Delta 12

Plasmid

#36439

Depositor

Edward Chan

Publication

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TNRC6A Delta 12 (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 896-1219 only

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
GST-SEPT5 S327A

Plasmid

#27271

Depositor

Harish Pant

Publication

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Tags

GST

Expression

Bacterial

Mutation

S327A

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pCMV5 MYC-Smurf2 (FF29/30A, Y459A) C716A

Plasmid

#24607

Depositor

Jeff Wrana

Publication

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A, Y459A, C716A

Available since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
HOXB13 (human) HIS-tag pET

Plasmid

#8543

Depositor

Corey Largman

Publication

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Western and by transcription/tra…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSKB3.MPN348

Plasmid

#11437

Depositor

Sung-Hou Kim

Publication

Chen et al Proteins. 2004 Sep 1. 56(4):839-43.

Insert

methenyltetrahydrofolate synthetase

Tags

6x His and TEV cleavage site

Expression

Bacterial

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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MTK1_023

Plasmid

#123685

Purpose

Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1- B1 insulator

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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