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pSBK.009

Plasmid

#187209

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCytERM-miRFP670nano3

Plasmid

#184674

Purpose

Signal anchor of rabbit cytochrome p450 and miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

CytERM-miRFP670nano3

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-MCP1

Plasmid

#184341

Purpose

Express yeast MCP1 in Expi293F cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Yeast MCP1 (MCP1 Budding Yeast)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0010

Plasmid

#172774

Purpose

Level 0 module containing NT1_CTPSSU

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0011

Plasmid

#172775

Purpose

Level 0 module containing NT2_CTPSSU

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-owl-monkey-retroCHMP3-HA

Plasmid

#154249

Purpose

expresses owl monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP1B 1-199

Plasmid

#115326

Purpose

Expresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-Sirius

Plasmid

#175825

Purpose

Express Sirius with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-KRasCT

Plasmid

#175833

Purpose

Express EGFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Venus-KRasCT

Plasmid

#175834

Purpose

Express Venus with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-iRFP

Plasmid

#175830

Purpose

Express iRFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Myc-MBP

Plasmid

#175103

Purpose

Lentiviral expression of Myc-tagged MBP

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

MBP

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-Dendra2

Plasmid

#169216

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJEC626

Plasmid

#174367

Purpose

Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23106

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC1617 pAAV SYN1 Nuc-EYFP

Plasmid

#135567

Purpose

An AAV vector expressing a neuronally expressed nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

empty
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

hSYN1

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

Plasmid

#169467

Purpose

Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp14D-EGFP

Plasmid

#165125

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp14D (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCcnb2

Plasmid

#162794

Purpose

Ccnb2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb2 shRNA (Ccnb2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shPrc1

Plasmid

#160959

Purpose

Prc1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Prc1 shRNA (Prc1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shMki67

Plasmid

#160958

Purpose

Mki67 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Mki67 (Mki67 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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