We narrowed to 22,921 results for: crispr

1. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101107

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   BDKRB2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PABE-1

   Plasmid

   #115622

   Purpose

   Targeted A to G in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Li et al Genome Biol. 2018 May 29;19(1):59. doi:

   Insert

   NLS-wtTadA-TadA7.10-nCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRRNA-G20-R20

   Plasmid

   #101822

   Purpose

   Repress mCherry and GFP independently, with different strengths

   Depositor

   Sven van Teeffelen, David Bikard

   Article

   Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

   Insert

   crRNAs targeting GFP and mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Spy 1049

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. A3F-Cas9n-UGI

   Plasmid

   #119138

   Purpose

   Base editor made from APOBEC3F

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3F-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. A3C-Cas9n-UGI

   Plasmid

   #119136

   Purpose

   Base editor made from APOBEC3C

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3C-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSDMA66

   Plasmid

   #128355

   Purpose

   gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

   Depositor

   James Fraser

   Article

   Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

   Insert

   Noureothricin (NAT)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   ACT1

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFH51

   Plasmid

   #128213

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 7xNLS-pMJ915v2-sfGFP

   Plasmid

   #88922

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAIO-AsCpf1

   Plasmid

   #137846

   Purpose

   AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   AsCpf1-NLS-FLAG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCF711

    Plasmid

    #121662

    Purpose

    U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. A3G-Cas9n-UGI

    Plasmid

    #119139

    Purpose

    Base editor made from APOBEC3G

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3G-Cas9 nickase-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOPO636

    Plasmid

    #195299

    Purpose

    pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    TnsABCQ operon of McCAST (Tn7575)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    IPTG inducible lac promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C-12-BE

    Plasmid

    #135040

    Purpose

    C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9

    Depositor

    Tian-Lin Cheng, Zilong Qiu

    Article

    Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

    Insert

    LjCDA1L2_1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 with EF-1alpha intron

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p202_LTJ_sgRNACD45.2_R1

    Plasmid

    #82672

    Purpose

    sgRNA targeting murine CD45.2 region 1. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD45.2 region 1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRRNA-G0-R0

    Plasmid

    #101803

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSDMA67

    Plasmid

    #128356

    Purpose

    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Insert

    Noureothricin (NAT)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    ACT1

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. N-8-BE

    Plasmid

    #135023

    Purpose

    C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9

    Depositor

    Tian-Lin Cheng, Zilong Qiu

    Article

    Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

    Insert

    LpCDA1L1_1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 with EF-1alpha intron

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDYSCKO

    Plasmid

    #120263

    Purpose

    Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    DYSCKO cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    SNR52

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMLS714

    Plasmid

    #188886

    Purpose

    MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCF713

    Plasmid

    #121665

    Purpose

    U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge