19,835 results

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pLVX-EGFP-C3-cenexin

Plasmid

#73334

Purpose

Lentiviral vector encoding cenexin with an N-terminal EGFP tag

Depositor

Manuel Thery

Insert

cenexin/ODF2 (ODF2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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EF13E

Plasmid

#42942

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

EN- Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

AgeI-BclI fragment swapped with nt 1927-3708 from…

Promoter

CMV MIE, L1 5'UTR

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only
5xNF-kb_RE::RedF

Plasmid

#124530

Purpose

NFKB RedFirefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

NF-κβ RedF reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTN4

Plasmid

#174212

Purpose

Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Inserts

AtTIR1(F79G)::mRuby (TIR1 Nematode)
GFP

Use

CRISPR and Cre/Lox

Tags

mRuby

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGE010

Plasmid

#71536

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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NUMB2-eGFP-M107K

Plasmid

#37803

Depositor

Rolf Bjerkvig

Article

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 2 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Promoter

pCMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pSico PGK puro

Plasmid

#11586

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro

Plasmid

#141369

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp4 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro

Plasmid

#141392

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9b

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-Wnt1

Plasmid

#35905

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG2LMKOSimO

Plasmid

#20866

Depositor

Keisuke Kaji

Article

Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

Insert

c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange (Myc Mouse)

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pBR.DT-A

Plasmid

#35955

Depositor

Jacqueline Lees

Article

Danielian et al Oncogene. 2008 Nov 20;27(51):6561-70. Ep

Insert

diphtheria toxin A

Use

Bac recombineering

Promoter

PGK

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC 1-1941

Plasmid

#16510

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Expression

Mammalian

Mutation

Codon 1941 truncation

Available Since

April 30, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GFP

Plasmid

#64545

Purpose

AAV-mediated expression of GFP under the CaMKII promoter.

Depositor

Edward Boyden

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-BTK

Plasmid

#23918

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BTK (BTK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pHYRS52

Plasmid

#31122

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

Ulp1 (ULP1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

Ulp1(403-621)

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-5FLAG-KanMX6

Plasmid

#15983

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Type

Empty backbone

Tags

5xFlag

Expression

Bacterial

Available Since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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Rasgrf2-2A-dCre targeting vector

Plasmid

#61573

Purpose

Target a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Rasgrf2-2A-dCre (Rasgrf2 P1 bacteriophage, Mouse)

Use

Cre/Lox and Mouse Targeting

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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lenti CAG-FLAG-dCas9-VPR

Plasmid

#114197

Purpose

Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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ATG-2460

Plasmid

#108713

Purpose

EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)

Depositor

Keith Wood

Article

Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

Insert

CBR2opt-copGFP fusion

Use

Lentiviral

Tags

CBR2opt fused to copGFP

Mutation

Promega used directed evolution to create CBR (Cl…

Promoter

elongation factor-1

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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