We narrowed to 19,441 results for: Tec
-
Plasmid#138155PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10APromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-niCPE
Plasmid#213747PurposeFor circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cellsDepositorInsertMCP-nLbCas12a-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R, R1138A in LbCas12aPromoterCMVAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT281
Plasmid#122611Purposeexpress evoAPOBEC1-BE4max in mammalian cellsDepositorInsertevoAPOBEC1-BE4max
ExpressionMammalianMutationE4K H109N H122L D124N R154H A165S P201S F205SAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD
Plasmid#190089PurposeExpresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD
Tags6xHis and MBPExpressionBacterialAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME270
Plasmid#238067PurposeExpresses ME-ABE ABE7.10-HRHSK with SpCas9 (NG)DepositorInsertTadA*7.10(L36H/L51R/Y123H/C146S/N157K)-m_SpnCas9(D10A)
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationTadA modifications include L36H, L51R, Y123H, C14…Available SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pG.Lantern-INS-SM50-GFP
Plasmid#135594PurposePropogation of Insulator-SM50 Promoter-GFP DNA for microinjection to visualize Insulator-GFP promoter activity in S. purpuratusDepositorInsertINS-SM50 promoter (SM50 )
UseIn vitro transcription (mrna synthesis)TagsGreen Lantern GFPPromoterCMVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-NLS-Cas9-6xHis
Plasmid#62934PurposeExpression of NLS-Cas9-6xHis in bacterial cellsDepositorInsertNLS-Cas9-6xHis
Tags6xHis tag and NLSExpressionBacterialPromoterT7Available SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
DR274
Plasmid#42250Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcriptionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR; Zebrafish expressionExpressionWormPromoterT7Available SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGM1202
Plasmid#69615Purposeinducible gene expression in Streptomyces, C-terminal His-tag encoding sequenceDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
Available SinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only