162,309 results

1. CMV-GFP-Kir2.1

   Plasmid

   #107184

   Purpose

   mammalian expression of Kir2.1

   Depositor

   Bikash Pattnaik

   Insert

   KCNJ2 (KCNJ2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-PelB-6H-Ag43-160N-sfGFP

   Plasmid

   #172886

   Purpose

   Display sfGFP protein on E. coli cell surface

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   SfGFP
   Ag43, partial (flu )
   

   Use

   Tags

   HHHHHH and TEV recognition site (ENLYFQG)

   Expression

   Bacterial

   Mutation

   Deleted 1-160 (N terminal)

   Promoter

   T7 Promoter

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. bZiPro

   Plasmid

   #86846

   Purpose

   express NS2B-NS3 protease in E.coli

   Depositor

   Dahai Luo

   Article

   Zhang et al Science. 2016 Dec 8. pii: aai9309.

   Inserts

   NS3 1-177
   NS2B45-96
   

   Use

   Tags

   a hexahistidine tag and a thrombin cleavage site

   Expression

   Bacterial

   Mutation

   NS2B cofactor region (residues 45–96) and NS3 pro…

   Promoter

   T7

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. C278

   Plasmid

   #174356

   Purpose

   For introducing multiple copies of OsTIR1 with the piggyBac system

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   OsTIR1
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKS004 - pCAGGS-3XFLAG-CTCF-eGFP

   Plasmid

   #156438

   Purpose

   For transient expression of mouse CTCF tagged with eGFP

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse CTCF (Ctcf Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. ACE2

   Plasmid

   #164219

   Purpose

   Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   silent mutation to remove internal EcoRI site (GA…

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCM189-ParB1::mCherry

   Plasmid

   #87253

   Purpose

   Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   ParB1
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   pBAD/tetO

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. NUDT3A

   Plasmid

   #42348

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   NUDT3A (NUDT3 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pMyNT

   Plasmid

   #42191

   Depositor

   Matthias Wilmanns, Annabel Parret

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   ColE1

   Available since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-TetOne-Puro-p21

    Plasmid

    #171122

    Purpose

    Doxycycline inducible expression of p21

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    p21 (CDKN1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209729

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG/GFP

    Plasmid

    #139980

    Purpose

    expresses GFP under the promoter of CAG. Transgene flanked by AAV2 ITR

    Depositor

    William Pu

    Article

    Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

    Insert

    Green fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+) mCherry

    Plasmid

    #176301

    Purpose

    Expresses mCherry mRNA from CMV promoter

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15-UbE2D1

    Plasmid

    #15782

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
    

    Use

    Tags

    6x His, T7 tag, and Xpress tag (enterokinase clea…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. Alpha-Synuclein-His

    Plasmid

    #186802

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    Alpha-Synuclein-His (SNCA Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_KLF7_P2A_Hygro_Barcode

    Plasmid

    #120493

    Purpose

    Barcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF7 (KLF7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-cT3G-cERP2-HDAC2

    Plasmid

    #222553

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    HDAC2 (HDAC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGBacmam-NGS Wnt-Fc

    Plasmid

    #159629

    Purpose

    Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd7/8 subtype NGS Wnt Fc
    

    Use

    Tags

    Fc tag and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. ER-mit RspA-splitFAST (lentiviral plasmid)

    Plasmid

    #233604

    Purpose

    Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-splitFAST
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV/SV40

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-dCas13-inactive M3M14nes

    Plasmid

    #157855

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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