We narrowed to 22,776 results for: his

Showing: 11521 - 11540 of 22776 results

Results per page:

Sort results by:

hnRNPA1-FLAG-IRES-eGFP

Plasmid

#234619

Purpose

Mammalian expression of full-length hnRNPA1 with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C-terminal FLAG tag, co-expressing with eGFP via …

Promoter

CMV

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-Synaptojanin-1-145

Plasmid

#223563

Purpose

Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

Depositor

Pietro De Camilli

Article

Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

SYNJ1 (SYNJ1 Human)

Tags

His and mCherry

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV GFAP jGCaMP7b

Plasmid

#171118

Purpose

Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

Depositor

Thomas Oertner

Article

Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

Insert

jGCaMP7b

Use

AAV

Tags

6 His Tag, T7 Tag, and Xpress Tag

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-32a(+)-TGFB1m

Plasmid

#120286

Purpose

Produce human recombinant mTGFB1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

monomeric Transforming Growth Factor-β1

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promotor

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CLuc-FKBP

Plasmid

#31184

Depositor

David Piwnica-Worms

Article

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

C-terminus of Firefly Luciferase and FK506-binding protein 12 (FKBP1A Human)

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDESTmycDICER

Plasmid

#19873

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

dicer 1, ribonuclease type III (DICER1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
HAP40 1-371

Plasmid

#124060

Purpose

Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HAP40 (F8A1 Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Promoter

polyhedrin

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFE-Rs-FI-prk

Plasmid

#162697

Purpose

Expresses R. spaeroides Form IC rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

R. spaeroides Form IC rubisco and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pLEGFP-Y705F-STAT3

Plasmid

#71445

Purpose

Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

Y705F

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hHSF1 RNAi resistant

Plasmid

#118347

Purpose

This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

RNAi resistance mutations (does not change amino …

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-32a(+)-TGFB3m

Plasmid

#120289

Purpose

Produce human recombinant mTGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

monomeric Transforming Growth Factor-β3

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-AKAP79-AKAR4

Plasmid

#181957

Purpose

Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-AKAR4 (AKAP5 Human)

Tags

AKAP79, Cerulean, HIS tag, and cpVenus(E172)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-M13-TevC-IRES-SP6-Cre

Plasmid

#171620

Purpose

M13-TevC with Cre

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

M13-TevC-IRES-SP6-Cre

Use

AAV and Synthetic Biology

Tags

6x His

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pC001 - huLshC2C2-MBP for bacterial expression

Plasmid

#79150

Purpose

Expresses human codon-optimized LshC2c2 for purification in E. coli

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

C2c2

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Human, Rat, Bacillus phage PBS2)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTet-SNAP-Cep152

Plasmid

#136825

Purpose

Mammalian expression of the centrosomal protein Cep152 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep152 (CEP152 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-PKM2-WT

Plasmid

#122690

Purpose

Express His-tagged PKM2 (wild type) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-GATA4

Plasmid

#46030

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GATA4 (GATA4 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

R43Q, G50S and N197S

Promoter

tetO

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge