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pMSCV Cyclin D2 long-wt

Plasmid

#21646

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pSC-hTDG-PreE2

Plasmid

#52272

Purpose

Bacterial expression of human TDG C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

Ubc9-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-Flag

Plasmid

#67164

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

FLag

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pJW1510

Plasmid

#62364

Purpose

pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ubc6 tail anchor

Expression

Yeast

Mutation

L232H mutation compared to standard mVenus

Promoter

Sec63

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCZGY2844

Plasmid

#87760

Purpose

Punc-17b-PH::miniSOG, expression of membrane targeted PH-miniSOG in cholinergic motor neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-17b-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Punc-17b

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBbS8k-RFP_E93G

Plasmid

#109411

Purpose

RepA Mutant

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

repA

Tags

RFP

Expression

Bacterial

Mutation

E93G repA mutation to increase copy number

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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p3PA-Puro-iTk

Plasmid

#109067

Purpose

inserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selection

Depositor

Eric Lander

Article

Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

Type

Empty backbone

Expression

Mammalian

Promoter

EFS

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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f2H ∂HD (272)

Plasmid

#24428

Depositor

Steven Rosen

Article

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF2 (SULF2 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

AA 418-716 deleted, 23AA N-terminal truncation

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
AIRAP 8: pCEFL.PSMB2.GST

Plasmid

#21798

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMB2 (Psmb2 Mouse)

Tags

GST

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pSMP-Suv39H2_1

Plasmid

#36344

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H2 (SUV39H2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
Beclin-HA S-A

Plasmid

#46994

Purpose

Expresses Beclin1 in mammalian cells

Depositor

Kunliang Guan

Article

Russell et al Nat Cell Biol. 2013 Jul;15(7):741-50. do

Insert

becn1

Tags

HA

Expression

Mammalian

Mutation

S14A

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
pSico Spry-2

Plasmid

#14809

Depositor

Tyler Jacks

Article

Shaw et al Genes Dev. 2007 Mar 15. 21(6):694-707.

Insert

Sprouty-2 shRNA (Spry2 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
C-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45222

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

C-term Hec1-targeted Aurora B FRET sensor

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pQE60 Trx1 CCAAA

Plasmid

#98402

Purpose

Bacterial expression vector for Trx1 CCAAA

Depositor

Tobias Dick

Article

Schwertassek et al EMBO J. 2007 Jul 11;26(13):3086-97. Epub

Insert

Trx1 CCAAA (TXN Human)

Expression

Bacterial

Mutation

C62A, C69A, C73A

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-C1

Plasmid

#54854

Purpose

Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

tdminiSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBbS8k-RFP_R43W

Plasmid

#109416

Purpose

RepA Mutant

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

repA

Tags

RFP

Expression

Bacterial

Mutation

R43W repA mutation to increase copy number

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-34

Plasmid

#119998

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Apr)

Plasmid

#110083

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pVHed02

Plasmid

#82352

Purpose

Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmid

Depositor

Richard Murray

Article

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

mKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6_Pfrm_BsMdh_BmHps_BmPhi

Plasmid

#129109

Purpose

Expresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter

Depositor

Eleftherios Terry Papoutsakis

Article

Rohlhill et al ACS Synth Biol. 2017 Aug 18;6(8):1584-15

Insert

BsMDH BmHPS BmPHI

Expression

Bacterial

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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