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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 11541 - 11560 of 21719 results

  6. gNS2B47NS3 L30SF31S

    Plasmid
    #155316
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    L30 to S, F31 to S
    Promoter
    M13
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155316-plasmid-map-sequence-id-311228

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  7. Cx43K258stop-msfGFP

    Plasmid
    #69023
    Purpose
    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Mutation
    delete codons 258-381 and fuse in frame to monome…
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69023-plasmid-map-sequence-id-124708

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  9. LSB-hsa-miR-181a-5p

    Plasmid
    #103276
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-181a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-181a-5p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103276-plasmid-map-sequence-id-195601

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  14. pNSSCP

    Plasmid
    #183188
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183188-plasmid-map-sequence-id-366663

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  16. pRCPam

    Plasmid
    #195861
    Purpose
    Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Insert
    Amber Chromogenic Protein on pRCPam plasmid
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Gln62X
    Promoter
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195861-plasmid-map-sequence-id-384172

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  19. Ana GvpC-SpyTag-His in pET28a

    Plasmid
    #85737
    Purpose
    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (fused to SpyTag and His-tagged)
    Use
    Tags
    His-tag and SpyTag
    Expression
    Bacterial
    Mutation
    Added an extra glycine after the methionine at th…
    Promoter
    T7
    Available since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    85737-plasmid-map-sequence-id-173841

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Showing: 11541 - 11560 of 21719 results