Addgene

We narrowed to 4,082 results for: Erf

Showing: 1141 - 1160 of 4082 results

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pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

Plasmid

#133334

Purpose

for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PRKCA

Plasmid

#67115

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PRKCA Protein kinase C alpha type (PRKCA Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pBS NL4-3 envFS eGFP Δgag/pol

Plasmid

#101343

Purpose

Deletion from the start of gag until 229 bases before the cPPT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta GagPol

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-scramble

Plasmid

#62285

Purpose

expression of scramble sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

scramble sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_EGFR

Plasmid

#183285

Purpose

All-in-One CRISPRko system with a guide RNA that targets EGFR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

EGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_SRC

Plasmid

#183322

Purpose

All-in-One CRISPRko system with a guide RNA that targets SRC gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

SRC

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ABL2

Plasmid

#183268

Purpose

All-in-One CRISPRko system with a guide RNA that targets ABL2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ABL2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ABL1

Plasmid

#183267

Purpose

All-in-One CRISPRko system with a guide RNA that targets ABL1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ABL1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a - SodB

Plasmid

#89613

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase B

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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IFI16-(515-710)-HinB

Plasmid

#35053

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(515-710)-HinB (IFI16 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains HinB domain: aa 515-710

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
pEN_hU6miR-Luc-A

Plasmid

#25756

Purpose

Entry vector with human U6 promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC3

Plasmid

#183299

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ESR1

Plasmid

#183288

Purpose

All-in-One CRISPRko system with a guide RNA that targets ESR1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ESR1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT1

Plasmid

#183282

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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GTH-g

Plasmid

#81104

Purpose

Gene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

sfGFP

Use

Cre/Lox

Tags

sfGFP

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1B

Plasmid

#183338

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1B

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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Pr-deCFP-MGapt

Plasmid

#67740

Purpose

OR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deCFP-MGapt-T500

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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IFI16-(192-393)-HinA

Plasmid

#35052

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains HinA domain: aa 192-393

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 PPARG

Plasmid

#67099

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PPARG (PPARG Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pALPS_3'LTR GFP@gag start SFFV tat

Plasmid

#101338

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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