We narrowed to 161,982 results for: addgene

1. pLenti-cytoMetGFP

   Plasmid

   #37561

   Depositor

   David Rimm

   Insert

   MET (MET Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   only contains cytoplasmic domain amino acids K956…

   Promoter

   CMV

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX302_CdTag-HA_EmptyVector

   Plasmid

   #221465

   Purpose

   Gateway backbone for C-terminal dTag cloning

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG2IG

   Plasmid

   #122292

   Purpose

   Overexpression control

   Depositor

   Yukiko Gotoh

   Article

   Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 6PBG

   Plasmid

   #162263

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   COPA (COPA Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. ETV5

   Plasmid

   #73234

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ETV5 (ETV5 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00178:Ets 368-450

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBABEneo-H-RAS_V12

   Plasmid

   #39525

   Depositor

   Julian Downward

   Insert

   HRAS V12 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V12 = G12V

   Promoter

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pBS35

   Plasmid

   #83797

   Purpose

   Useful for tagging yeast proteins with C-terminal mCherry tag; hygromycin-B selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   mCherry
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-tkd2MetGFP

   Plasmid

   #37562

   Depositor

   David Rimm

   Insert

   MET (MET Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   only contains cytoplasmic domain amino acids L115…

   Promoter

   CMV

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Amber C1

   Plasmid

   #27797

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Insert

   mAmber C1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRIM33 (TRIM33B-c100)

    Plasmid

    #98243

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TRIM33B (TRIM33 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00628:PHD 889-932, pfam00439:Bromodomain 963-…

    Promoter

    T7

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS4

    Plasmid

    #83793

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 4QQ6

    Plasmid

    #61946

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SMN1:JMC047-F11:C221089 (SMN1 Human)
    

    Use

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. CYP11A1 (3N9Y)

    Plasmid

    #28119

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP11A1:HPC09N-C05:C214491 (CYP11A1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pBS10

    Plasmid

    #83795

    Purpose

    Useful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    Cerulean
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPD95.77-wVenus

    Plasmid

    #37465

    Depositor

    Hitoshi Sawa

    Article

    Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMBPcyto-TEV-BseRI

    Plasmid

    #157739

    Purpose

    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Use

    Tags

    His10 + MBP (cytoplasmic expression; no signal se…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBES2

    Plasmid

    #83800

    Purpose

    Useful for tagging yeast proteins with N-terminal Venus tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    Venus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUPD2:35S-TMV-Rep-MP

    Plasmid

    #106217

    Purpose

    Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)

    Depositor

    Tomáš Moravec

    Insert

    Infectious clone of Tobacco mosaic virus, 5' part
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6D1T (MBD1)

    Plasmid

    #119966

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    MBD1 (MBD1 Human)
    

    Use

    Tags

    GSTtag, 6xHIS and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. 6WNX

    Plasmid

    #162270

    Purpose

    Bacterial Expression

    Depositor

    Cheryl Arrowsmith

    Insert

    FBXW11 (FBXW11 Human)
    

    Use

    Baculovirus expresssion

    Tags

    6xHis-TEV

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge