2,294 results

1. mTFP1-CathespinB-6

   Plasmid

   #55467

   Purpose

   Localization: Lysosomes, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   CathespinB
   

   Tags

   mTFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. FUap97GW (C4)

   Plasmid

   #74020

   Purpose

   lentiviral expression of SAP97alpha-EGFP fusion

   Depositor

   Robert Malenka, Weifeng Xu, Oliver Schluter

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Insert

   SAP97 alpha
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSIL-eGFP-shACTN2

   Plasmid

   #52677

   Purpose

   shRNA against α-actinin-2 with eGFP transfection marker

   Depositor

   Johannes Hell

   Article

   Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

   Insert

   ACTN2 (Actn2 Rat)
   

   Use

   RNAi

   Tags

   CMV-EGFP

   Promoter

   U6

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. SyGCaMP5G

   Plasmid

   #162519

   Purpose

   Synaptically targeted GCaMP5G

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GCaMP5G
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. KCC2-pHext

   Plasmid

   #104076

   Purpose

   To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

   Depositor

   Igor Medyna

   Article

   Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00

   Insert

   KCC2 (b isoform) (Slc12a5 Rat)
   

   Tags

   pHluorine tag inserted in the second putative tra…

   Expression

   Mammalian

   Mutation

   EcoR1 site in rat KCC2 was removed by silent muta…

   Promoter

   UbC

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-hPGK-mCherry-SynaptoZip

   Plasmid

   #177317

   Purpose

   Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

   Depositor

   Antonio Malgaroli

   Article

   Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

   Insert

   mCherry-SynaptoZip (Vamp2 Rat)
   

   Use

   Lentiviral

   Tags

   Myc and mCherry

   Expression

   Mammalian

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. FCIV1-GR

   Plasmid

   #72701

   Purpose

   Lentiviral expression of rat GR (2nd generation)

   Depositor

   Freddy Jeanneteau

   Article

   Lambert et al Mol Cell Biol. 2013 Sep;33(18):3700-14.

   Insert

   GR (Nr3c1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-Venus

   Expression

   Mammalian

   Promoter

   ubiquitin

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

8. rNrx1beta(47-299)

   Plasmid

   #20173

   Depositor

   Axel Brunger

   Article

   Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

   Insert

   Neurexin 1 beta (Nrxn1 Rat)
   

   Tags

   6X His tag

   Expression

   Insect

   Mutation

   no splice site

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

9. CD200RCd4d3+4-bio

   Plasmid

   #32406

   Purpose

   A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

   Depositor

   Gavin Wright

   Article

   Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

   Insert

   CD200 Receptor extracellular domain (Cd200r1 Rat)
   

   Tags

   Cd4d3+4 and biotinylation peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-Syn-H2B-jGCaMP8s-WPRE

    Plasmid

    #176749

    Purpose

    AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    H2B-jGCaMP8s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    synapsin

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mVenus-Lysosomes-M-20

    Plasmid

    #56618

    Purpose

    Localization: Lysosome Membrane, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    LAMP1 M
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    R8 silent mutation; CGT

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pOC1 Gria1-GFP-FRB KI

    Plasmid

    #183428

    Purpose

    CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP-FRB donor
    

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOC2 Dlg4-Halo-FKBP KI

    Plasmid

    #183435

    Purpose

    CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo-FKBP donor
    

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI.neo-rPAM1-M314I

    Plasmid

    #145802

    Purpose

    expresses catalytically inactive rat PAM-1 in mammalian cells

    Depositor

    Richard Mains, Betty Eipper

    Article

    Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

    Insert

    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    

    Expression

    Mammalian

    Mutation

    changed methionine 314 to isoleucine to inactivat…

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-SV40-Galpha-i1 ONE-GO

    Plasmid

    #204328

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    SV40 Galpha-i1 ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET14b PHAS-I F113A

    Plasmid

    #15680

    Depositor

    John Lawrence

    Article

    Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

    Insert

    PHAS-I F113A (Eif4ebp1 Rat)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F113A mutation.

    Available Since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-Sec22b-P33-shR

    Plasmid

    #208359

    Purpose

    Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    Sec22b (Sec22b Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    four silent mutations (shRNA resistance), 33 aa p…

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQZ210

    Plasmid

    #106414

    Purpose

    Co-expresses trans SNARE complex with pDC183 in E.coli

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

    Inserts

    Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
    Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Fragment 191-256 , Codon optimization and Fragmen…

    Promoter

    T7

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-H1-SGIPZ_NF1 sh1miR

    Plasmid

    #119206

    Purpose

    knockdown of NF1

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    sh1miR targeting NF1 (NF1 Rat, Human, Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cherry-Myo1b

    Plasmid

    #187366

    Purpose

    Expresses rat myosin 1b isoform b domain labelled with Cherry

    Depositor

    Evelyne Coudrier

    Article

    Iuliano et al J Cell Biol. 2018 Jun 4;217(6):2033-2046

    Insert

    myosin 1b isoform b (Myo1b Rat)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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