We narrowed to 23,480 results for: CHI

1. pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174881

   Purpose

   Targeting vector for generating Flnc STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Flnc
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174885

   Purpose

   Targeting vector for generating Usp5 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Usp5
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174886

   Purpose

   Targeting vector for generating Wnk1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Wnk1
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro

   Plasmid

   #169665

   Purpose

   Tier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK / RPBSA

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

   Plasmid

   #169664

   Purpose

   Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / RPBSA

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJJGL003

   Plasmid

   #180607

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   DpbCasX with R3 loop
   

   Use

   CRISPR

   Tags

   10x His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB1C3_mTq-Tat (c-plasmid)

   Plasmid

   #127635

   Purpose

   Encodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

   Insert

   mTurquoise2
   

   Use

   Synthetic Biology

   Tags

   RNA binding peptide: PCP and RNA binding peptide:…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29a-hNABP1NhNABP2C

   Plasmid

   #128312

   Purpose

   Express chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   chimeric protein hNABP1N+hNABP2C (NABP1, NABP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   chimeric protein hNABP1N+hNABP2C

   Promoter

   T7

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-ecDHFR-dCys-T46C

   Plasmid

   #110543

   Purpose

   Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

   Insert

   ecDHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T46 mutated to C46, C85 mutated to A85, C152 muta…

   Promoter

   T7

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22b-ecDHFR-dCys-L54C

    Plasmid

    #110542

    Purpose

    Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L54 mutated to C54, C85 mutated to A85, C152 muta…

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b-ecDHFR-D27S/Y100F

    Plasmid

    #110827

    Purpose

    Bacterial expression of E.coli DHFR D27S/Y100F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D27 mutated to S27, Y100 mutated to F100

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMYA5T2/pDEST17

    Plasmid

    #98118

    Purpose

    Express CMYA5 (aa 4039 – 4069) Leu allele (4063) witha N-terminal 6x-His tag in bacteria

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    rs10043986 (NP_705838.3:p.Pro4063Leu)

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. CMYA5T/pACT2

    Plasmid

    #97209

    Purpose

    CMYA5 (aa 4039 – 4069) Leu allele (4063) in a GAL4 AD vector, used for yeast-two hybrid

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    rs10043986 (NP_705838.3:p.Pro4063Leu)

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pNC2

    Plasmid

    #72438

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg

    Promoter

    T7 and n/a

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZS1[RbsR-T]

    Plasmid

    #60751

    Purpose

    Contains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Insert

    RbsR-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Chimeric LacI/GalR repressor with the TAN DBD and…

    Promoter

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHBD00142

    Plasmid

    #34296

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8819 (achi Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL

    Plasmid

    #233628

    Purpose

    Expression of bicistronic reporter with TOP motif

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    Promoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX Cis1-SH2

    Plasmid

    #46417

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Cis1 (SOCS1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 90-214

    Promoter

    Tac

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX Socs3-SH2

    Plasmid

    #46506

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs3 (SOCS3 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 22-142

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Socs1-SH2

    Plasmid

    #46504

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs1 (SOCS1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-166

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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