We narrowed to 10,632 results for: t7

1. pGEMHE-mCherry-mTrim21

   Plasmid

   #105522

   Purpose

   T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mCherry

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a_SUMO_AcCas12n

   Plasmid

   #203808

   Purpose

   pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   SUMO_AcCas12n
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKSJ376

   Plasmid

   #103065

   Purpose

   Expresses colicin E1 with R domain deletion and ImmE1

   Depositor

   Alan Finkelstein, Karen Jakes

   Article

   Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

   Inserts

   colicin E1 immunity protein (pColE1_26, imm E. coli)
   cea/colicin E1
   

   Expression

   Bacterial

   Mutation

   R (receptor-binding domain) deletion in colicin E…

   Promoter

   T7 lac (from pET) and T7 lac (from pET52-b)

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBP-T7_RBS-His6-Thrombin

   Plasmid

   #72979

   Purpose

   T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7+RBS+His6+thrombin
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA-Flag-H3.3-polyA

   Plasmid

   #59780

   Purpose

   Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   H3F3B (H3-3B Human)
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJAB988

   Plasmid

   #133920

   Purpose

   miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

   Depositor

   Christopher Voigt

   Article

   Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.

   Insert

   miniICE-tet(M)cat(PC194)_PT7-GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p2,0_3M5M_eGFP

   Plasmid

   #135474

   Purpose

   Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   Marburg virus minigenome, eGFP reporter
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   T7

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p2,0_3M5M_luciferase

   Plasmid

   #135475

   Purpose

   Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   Marburg virus minigenome, luciferase reporter
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   T7

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEMHE-mEGFP-mTrim21

   Plasmid

   #105519

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Promoter

   T7

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MC177VSG221RNAi

    Plasmid

    #59730

    Purpose

    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

    Depositor

    Gloria Rudenko

    Article

    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

    Insert

    VSG221 RNAi fragment
    

    Use

    RNAi

    Promoter

    opposing T7 promoters

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJBL715

    Plasmid

    #140385

    Purpose

    Expresses MphR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-MphR-T
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28b-hUba2-His

    Plasmid

    #53117

    Purpose

    expression of full length human Uba2 with Cterminal His-tag in E.coli

    Depositor

    Frauke Melchior

    Article

    Werner et al Methods Mol Biol. 2009;497:187-99. doi:

    Insert

    UBA2 (UBA2 Human)
    

    Tags

    His and T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJBL723

    Plasmid

    #140393

    Purpose

    Expresses HucR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-HucR-T
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNP1 sfGFP toehold switch

    Plasmid

    #107355

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold 7 + sfGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJBL711

    Plasmid

    #140381

    Purpose

    Expresses OtrR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-OtrR-T
    

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJBL713

    Plasmid

    #140383

    Purpose

    Expresses CtcS protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CtcS-T
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPN-mKate (PT7LacO-PN-mKate)

    Plasmid

    #191126

    Purpose

    Expression of protein N tagged with mKate from a T7-lac inducible promoter

    Depositor

    Ariel Lindner

    Article

    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

    Insert

    Protein N
    

    Use

    Synthetic Biology

    Tags

    mKate

    Promoter

    T7-lac inducible promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJBL701

    Plasmid

    #140371

    Purpose

    Expresses TetR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-TetR-T
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEMHE-GST

    Plasmid

    #171486

    Purpose

    T7 promotor drives in vitro transcription of GST mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    GST
    

    Use

    Pgehme

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6T7

    Plasmid

    #71462

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    human U6/phage T7

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge