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pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

Plasmid

#134337

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Efa

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-ERR3 S45A

Plasmid

#37849

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

Estrogen-related receptor gamma (Esrrg Mouse)

Tags

HA

Expression

Mammalian

Mutation

changed Serine 45 to Alanine (AGC to GCC)

Promoter

SV40

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-gp78c/2767

Plasmid

#11429

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78c (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pJM019

Plasmid

#134158

Purpose

Retroviral plasmid expressing GFP PSMA3

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

PSMA3 (PSMA3 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-UBE2S-UBD

Plasmid

#66713

Purpose

Expresses the UBE2S-UBD fusion protein in E. coli

Depositor

David Komander

Article

Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

Inserts

e2-epf (UBE2S )
isot (USP5 )

Tags

GST-3C

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102797

Purpose

Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-USP11

Plasmid

#78849

Purpose

To purify GST-tagged recombinant USP11 protein

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

Insert

USP11 (USP11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-KG-Itch/2793

Plasmid

#11434

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

Itch (Itch Mouse)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST_5xUAS:EB3-mScarlet-I

Plasmid

#192358

Purpose

EB3 tagged with mScarlet-I, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

EB3 (MAPRE3 Human)

Tags

mScarlet-I

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIH-myc-hTAZ

Plasmid

#32841

Depositor

Kunliang Guan

Article

Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

Insert

TAZ (WWTR1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV/MSV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only
pLX317-VRK2

Plasmid

#199641

Purpose

Expresses VRK2

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK2 (VRK2 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-OSBP

Plasmid

#67664

Purpose

Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-OSBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Vitiosangium-bGSDM-thrombin

Plasmid

#222099

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Vitiosangium-bGSDM-thrombin ORF

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Nostoc-bGSDM-WELQ

Plasmid

#222100

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Nostoc-bGSDM-WELQ ORF

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-tdTomato-cNLS

Plasmid

#138532

Purpose

Lentiviral inducible expression of tdTomato with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

tdTomato

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE-CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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LLP773_pLenti-6xHRBKET-sgRNA

Plasmid

#211766

Purpose

Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)

Use

Lentiviral

Promoter

pCMV and U6

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLIC-His-RGS4

Plasmid

#204847

Purpose

Express His tagged-RGS4 in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

RGS4 (RGS4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mKate2-P2A-APEX2-ML1N*2

Plasmid

#67665

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1N*2

Expression

Mammalian

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone1

Plasmid

#162119

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCLJ-F2P-LoxP-PGK-PuroR-LoxP

Plasmid

#227502

Purpose

Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Prdm8 promoter

Use

AAV and Cre/Lox

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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