171,035 results

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DL033:cFos::eGFP-iPAK4

Plasmid

#177883

Purpose

eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

iPAK4

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

cFos

Available Since

July 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCIG2-Flag-mNuak1 WT

Plasmid

#168507

Purpose

For expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)

Depositor

Franck Polleux

Article

Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

Insert

Nuak1 (Nuak1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB533

Plasmid

#74872

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3 Enhancer Vector

Plasmid

#212938

Purpose

Firefly luciferase vector for investigating regions controlling transcription

Depositor

Debrya Groskreutz

Type

Empty backbone

Use

Luciferase

Mutation

WT

Available Since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTRE3G-EGFP-IRES(Neo)R

Plasmid

#158068

Purpose

eGFP under the TRE3G promoter, Neo resistance. For cloning GOI

Depositor

Adolfo Rivero-Muller

Article

Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

Insert

eGFP

Tags

eGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe bleo human PPAR gamma 2

Plasmid

#11439

Depositor

Ronald Kahn

Insert

PPAR gamma 2 (PPARG Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pGL-p21UTR

Plasmid

#20014

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR (Cdkn1a Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pBad-LgBiT-PhoCl1-SmBiT-MBP

Plasmid

#164034

Purpose

luciferase-based PhoCl screening construct

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

LgBiT-PhoCl1-SmBiT-MBP

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Promoter

araBad promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF0385

Plasmid

#143874

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMARCA4 (SMARCA4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYLL204

Plasmid

#53455

Purpose

Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

Depositor

Leroy Liu, Lisa Lyu

Article

Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

Insert

HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)

Tags

HA

Expression

Mammalian

Mutation

Y814F

Promoter

CMV

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO V5

Plasmid

#19445

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

V5

Expression

Mammalian

Mutation

V5 tag inserted to create new vector

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

Plasmid

#168305

Purpose

mediated knock-in of sgRNA precursor

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

SaKKH-EGFP-U6-sgRNA

Use

AAV

Expression

Mammalian

Promoter

EF1alpha, CBh

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBS L30 mRuby3-Rab18

Plasmid

#166431

Purpose

Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Insert

Rab18

Tags

mRuby3

Expression

Mammalian

Promoter

L30 weak promoter

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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ecAT3.10

Plasmid

#107215

Purpose

Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

Depositor

Mathew Tantama

Article

Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

Insert

ecAT3.10

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes1+2 f.l. WT

Plasmid

#158540

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pVAX1-BMP2

Plasmid

#137909

Purpose

Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

Depositor

Georg Feichtinger

Article

Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

Insert

BMP2 (BMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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Mortalin/GRP75 scFv [N52A/42]

Plasmid

#182091

Purpose

Mammalian Expression of Mortalin/GRP75 scFv. Derived from hybridoma N52A/42.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Mortalin/GRP75 (Mus musculus) (Hspa9 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hACE2

Plasmid

#145033

Purpose

Express human ACE2 with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

human ACE2 (ACE2 Human)

Tags

C9

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLminP_Luc2P_RE44

Plasmid

#90382

Purpose

Hedgehog - Gli1 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

GLI

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR/D_creERt2

Plasmid

#27321

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

creERt2

Use

Multisite gateway middle entry vector

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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