We narrowed to 13,064 results for: lic
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Plasmid#186523PurposeHyperfolder YFP fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertHyperfolder YFP
ExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCgCas9_recT
Plasmid#153337PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; KanrDepositorInsertCas9
UseCRISPRExpressionBacterialAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
ExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
ExpressionYeastPromoterPGK1Available SinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lyso-GA
Plasmid#209874PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP A
TagsLAMP1ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq)
Plasmid#230015PurposeExpresses anti-TNFalpha PAGER(Gq) in mammalian cellsDepositorInsertMT1-Ozoralizumab-TEVcs-ALFA-hM1D
UseAAVExpressionMammalianMutationW442A on hM1DPromoterCMVAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-cyto
Plasmid#113917PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
ExpressionMammalianPromoterCMV TetO2Available SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gi)
Plasmid#230019PurposeAAV vector expressing anti-mCherry PAGER(Gi) in neuronsDepositorInsertMT1-LaM6-TEVcs-ALFA-hM1D
UseAAVExpressionMammalianPromoterhSynAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-eGFP
Plasmid#176861PurposeExpresses cytosolic eGFP in astrocytesDepositorInserteGFP
UseAAVExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav-GA
Plasmid#209880PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1DepositorInsertddGFP A
TagsCAVIN1ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP-134/150TAG
Plasmid#174077PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
TagsHis-6ExpressionBacterialMutationD134TAG + N150TAGPromoterT7Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PM-B
Plasmid#209876PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddGFP B
TagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
epi-BE4max
Plasmid#135975PurposeExpresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication originDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
LD-GA
Plasmid#209862PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddGFP A
TagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-H-Luc_cyto
Plasmid#113929PurposePlasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRETDepositorInsertH-Luc
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
PM-GA
Plasmid#209877PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddGFP A
TagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRT801
Plasmid#68112PurposeIntegration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
oriT
attP
int
UseStreptomyces integrating vectorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEC2861 (pSpy1C-PgyrA_ffluc)
Plasmid#218511Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseMutationdeletion of Plac_mrfp cassettePromoterPgyrAAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
epi-BE4max-NG
Plasmid#135977PurposeExpresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication originDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
G555
Plasmid#216483PurposeExpression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.DepositorInsertplsB, plsC, cdsA, pssA
UseCell-free expressionPromoterT7 for each geneAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only