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AiP11408-pAAV-AiE0064h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1408)

Plasmid

#163500

Purpose

AiE0064h is an enhancer sequence designed to drive AAV-mediated transgene expression in Pvalb+ and Sst+ cortical interneurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Zhou-COVID-19-Spike

Plasmid

#161029

Purpose

Extracellular domain of SARS-CoV-2 spike protein that has been engineered so that it assembles pseudo virus more efficiently. Also contains portions of VSV-G for production of psuedovirus.

Depositor

Dapeng Zhou

Article

Tong et al Vaccines (Basel). 2021 Mar 22;9(3). pii:

Insert

Engineered COVID-19 Spike protein, VSV-G (S SAR-CoV-2, Vesicular stomatitis virus)

Expression

Mammalian

Mutation

Codon optimized

Available Since

Jan. 12, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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ePB-PURO-FLAG-METTL3-APPA

Plasmid

#160253

Purpose

For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

tight TRE promoter

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRG713_lexO-HO_LexA-TF_URA3MX

Plasmid

#154821

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AC50 pEGFPN1 anti-AMIGO-1 nanobody

Plasmid

#135225

Purpose

Mammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-AMIGO-1 (Mouse) recombinant llama nanobody AC50 (Amigo1 L. glama (llama))

Tags

6xHis-HA-EGFP

Expression

Mammalian

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-TAK1-K63A

Plasmid

#43933

Depositor

Bryant Darnay

Article

Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

Insert

Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

K63A

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSKC12 - DelQ

Plasmid

#110493

Purpose

Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Article

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Insert

NRAS 5'- DelQ (bp 30-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Mutation

Deletion of the first 29 base pairs of the NRAS 5…

Promoter

T7

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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Cherry-HA-KCC2

Plasmid

#104077

Purpose

To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

Depositor

Igor Medyna

Article

Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Tags

mCherry-HA tag

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

UbC

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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hsDCX-pHAT

Plasmid

#83928

Purpose

hsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

human Doublecortin isoform b (DCX Human)

Tags

6-His tag and Strep-TagII

Expression

Bacterial

Available Since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Plxna1-AP-His

Plasmid

#71996

Purpose

Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna1 (Plxna1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP-Kif5cmut

Plasmid

#71854

Purpose

mammalian expression of mutant GFP-Kif5c

Depositor

Michelle Peckham

Article

Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

Insert

Kif5c (Kif5c Rat)

Tags

EGFP

Expression

Mammalian

Mutation

T93N mutation and also a novel SpeI site (1652). …

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP3(307-574)

Plasmid

#71481

Purpose

Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Deleted amino acids 1-306 (catalytic domain only)

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only
mCardinal-H3-23

Plasmid

#56163

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H3 (H3C2 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
miR-34aM53

Plasmid

#50830

Purpose

luciferase reporter for human miR-34a

Depositor

Yun Bai, Xueqing Xu

Article

Li et al BMC Mol Biol. 2012 Jan 31;13:4.

Insert

miR-34A promoter M53 (MIR34A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

p53 binding site mutated (-39)

Promoter

miR34a

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only
pCA-G-intron-T

Plasmid

#36885

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pLXSN-H-Ras_V12

Plasmid

#39516

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pCS2 Smad1 EVE GM

Plasmid

#27997

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

Human Smad1 EVE Gsk3 mutant (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S463E S465E S210A T202A S191A S198A

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only
ATF4 18: RLuc.5’ATF4.GFP

Plasmid

#21864

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
608 pSG5L HA RB del ex4

Plasmid

#10730

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del exon4 (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

deletion of exon4

Available Since

Oct. 7, 2005

Availability

Academic Institutions and Nonprofits only
AAV-hSyn-RiboL1-jGCaMP8f

Plasmid

#169249

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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