We narrowed to 15,769 results for: LEA

1. pGBW-m4046773

   Plasmid

   #145708

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBW-m4046778

   Plasmid

   #145618

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGBW-m4046904

   Plasmid

   #145591

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pTH651-RLuc/minFLuc

   Plasmid

   #29697

   Depositor

   Tobias von der Haar

   Article

   Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

   Insert

   Firefly Luciferase
   

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Available Since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGC4785

   Plasmid

   #113409

   Purpose

   Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

   Depositor

   Adam Arkin, Guillaume Cambray

   Article

   Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

   Insert

   359_31121113_2
   

   Use

   Synthetic Biology

   Tags

   fused to codon deptimized sfGFP with Flexible lin…

   Expression

   Bacterial

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1-S-Luc_nucl

   Plasmid

   #113928

   Purpose

   Plasmid encoding the nuclear localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   S-Luc_nucl
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PHF13 (3O7A)

   Plasmid

   #28155

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PHD finger protein 13 (PHF13 Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   PHD-type zinc finger domain (residues 250-301.

   Available Since

   April 4, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

8. His8-TEV-PseH

   Plasmid

   #89725

   Purpose

   Expresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site

   Depositor

   Barbara Imperiali

   Article

   Zamora et al Methods Enzymol. 2017;597:145-186. doi:

   Insert

   PseH (pseH Campylobacter jejuni)
   

   Tags

   2xHis Gln 5xHis and TEV

   Expression

   Bacterial

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKF251

   Plasmid

   #52532

   Purpose

   Double-GFP reporter for DNA cleavage

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   Double-GFP
   

   Expression

   Insect

   Promoter

   ubiquitin

   Available Since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIRECT_37J

    Plasmid

    #91153

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:TALEN
    

    Use

    TALEN

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEG587

    Plasmid

    #40196

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (S458A) (RNAi resistant) (mex-5 Nematode)
    

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    S458A; exon 2 recoded to be RNAi-resistant

    Promoter

    4.4 kb mex-5 promoter fragment

    Available Since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pET21a - NDH

    Plasmid

    #89626

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    NAD(P)-dependent oxidoreductase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-SMVP-Cas9N-U6-gRNA M4

    Plasmid

    #80937

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA-FRT-FAK-FERM10

    Plasmid

    #59126

    Purpose

    FAK fret control sensor without kinase domain with 10 nm linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. mPapaya1-Nup50-N-10

    Plasmid

    #56355

    Purpose

    Localization: Nuclear Pore, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    Nup50 (NUP50 Human)
    

    Tags

    mPapaya1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pHL1756

    Plasmid

    #53036

    Purpose

    PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    GFP
    tetR
    

    Use

    Synthetic Biology

    Tags

    RBS(st3) and glgC leader

    Expression

    Bacterial

    Promoter

    pConNoHind and pConNoHindM12

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pEG585

    Plasmid

    #40192

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
    

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    S404A; exon 2 recoded to be RNAi-resistant

    Promoter

    4.4 kb mex-5 promoter fragment

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pStayGold

    Plasmid

    #243674

    Purpose

    Expresses StayGold fluorescent protein in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator

    Depositor

    Patrick Krysan

    Article

    De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.

    Insert

    StayGold fluorescent protein
    

    Expression

    Plant

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. L4872 VP64-intN in PolyTX-mNeonGreen

    Plasmid

    #244191

    Purpose

    Constitutive expression of VP64-intN split synTF component

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    Split synTF component VP64-intN
    

    Use

    Synthetic Biology

    Tags

    3xFLAG - nuclear localization sequence

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. RC00214-pcsb-35Sp-ccdB-mNG-OCSp-tdT

    Plasmid

    #209019

    Purpose

    Dual-fluorescence acceptor plasmid for one-step insertion of a 5' leader of interest between the CaMV35S promoter and mNeonGreen via BpiI. A tdTomato expression cassette serves as internal control.

    Depositor

    Stephen Long

    Inserts

    ccdB
    mNeonGreen
    tdTomato
    

    Expression

    Plant

    Mutation

    codon optimized for N. benthamiana

    Promoter

    CaMV35S and Octopine Synthase

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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