We narrowed to 31,400 results for: LIS
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Plasmid#211516PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterSV40 and minTKAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only
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pTXB1-TnY
Plasmid#163975PurposeIPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.DepositorInsertTnY
TagsMxe intein - Chitin-binding domainExpressionBacterialAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor lambda version
Plasmid#66073PurposeThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellsDepositorInsertGFP
Available SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
Plasmid#83485PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS4S
Plasmid#55170PurposeEmpty vector, integration at thrC, ampr, specrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxPromoternoneAvailable SinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-dALFANb(5mut)-mGreenLantern
Plasmid#219661PurposeGal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLanternDepositorInsertDestabilised ALFA nanobody (5 mutations) fused to mGreenLantern
ExpressionInsectAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3869 HA-E6-AP C833A
Plasmid#8649DepositorInsertE6-AP (UBE3A Human)
TagsHAExpressionMammalianMutationC833A; active site Cys mutated to encode AlaAvailable SinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
HSC1-GiP
Plasmid#58254PurposeThis self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassetteDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseRetroviralExpressionMammalianPromoterHuman EF1-alphaAvailable SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
SPDK3860
Plasmid#199245PurposeModified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) geneDepositorInsertModified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorPromoterpPEBVAvailable SinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPb-FKBP-post mGRASP
Plasmid#73146PurposeSmall fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.DepositorInsertsHRPb-FKBP-post mGRASP
TagsHA tagExpressionMammalianPromoterCAGAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI
Plasmid#115210PurposeExpresses AspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertAspCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hL1
Plasmid#89411Purposemammalian expression of hL1DepositorAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE-hygro-GFP-Crumbs3a WT
Plasmid#195217PurposeRetroviral Tet response vector containing GFP tagged Crumbs3aDepositorInsertCRB3A (CRB3 Human)
UseRetroviralAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-dALFANb(3mut)-mGreenLantern
Plasmid#219662PurposeGal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLanternDepositorInsertDestabilised ALFA nanobody (3 mutations) fused to mGreenLantern
ExpressionInsectAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
Available SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-Envelope protein
Plasmid#215700PurposeProduces the Chikungunya virus envelope protein E3-E2-6K-E1DepositorInsertCHIKV E3-E2-6K-E1
ExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Gad65P-eGFP-3XNLS-WPRE-pA
Plasmid#183804PurposeAAV vector to express nuclear localized eGFP from a Gad65 promoterDepositorInserteGFP-NLS
UseAAVTagsNLSAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE491
Plasmid#149292PurposeT-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2DepositorInsertp35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
ExpressionPlantPromoter35SAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1079A
Plasmid#83494PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)DepositorInsertLbu_C2c2_R1079A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1079APromoterT7Available SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEE531
Plasmid#149290PurposeT-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2DepositorInsertp35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
ExpressionPlantPromoter35SAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only