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Plasmid#144463PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only
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IDG_CLCC1_OE_1
Plasmid#161672PurposeOverexpresses CLCC1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCLCC1 (CLCC1 Human)
TagsV5ExpressionMammalianAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-N-TEV-Twin-Strep
Plasmid#202526PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneTagsTEV cleavable site and Twin-strep-tagExpressionMammalianPromoterCAGAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBUE411-GG
Plasmid#198232PurposeWheat GRF4-GIF1 chimera in pBUE411DepositorPublicationInsertGRF4-GIF1 chimera cassette
UseCRISPR; Plant binary vectorExpressionPlantPromoterUbiquitinAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV2cap
Plasmid#198014PurposeFor high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV2 cap
UseAAVExpressionMammalianMutationWTPromoterCMVAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3363
Plasmid#144839PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lsg-GBA-1 + 2
Plasmid#199276Purposenicking sgRNA to induce or correct GBA (c. 1226 A > G) mutation using PE3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGBA nick
ExpressionMammalianAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-mNeonGreen-CAMSAP3
Plasmid#191322PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flowDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmNeonGreen-CAMSAP3 (Camsap3 Mouse)
ExpressionMammalianAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZFP36L2
Plasmid#156149PurposeFor use in RBP tethering screenDepositorAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptideExpressionBacterialPromoterPhyper spankAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
UPB-stomach (UPB1)
Plasmid#180784PurposeUnique Projection Barcode (UPB1)-Stomach for Projection-seqDepositorInserthumanized ChR2-H134R (partial)
UseAAVExpressionMammalianPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
Plasmid#185451PurposeExpresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cellsDepositorAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA flag-Msi1
Plasmid#184029PurposeMsi1 clone with N-terminal flag tagDepositorAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9-POLD3
Plasmid#183194PurposeExpression cloneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPOLD3
UseCRISPRTagsCas9WTExpressionMammalianMutationn/aAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRedCmOcr
Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyExpressionBacterialPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-UXPS
Plasmid#172136Purposemammalian expression plasmid for a UDP/UTP sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUXPS
ExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-C-3xVHH05-DsRed
Plasmid#171580PurposeFor scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorPublicationInsert3xVHH05 (UBE2J1 Human)
ExpressionInsectAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFlox_N-Nipbl_BSD_FKBPF36V_EYFP
Plasmid#163882PurposeAcute Depletion of NIPBL-EYFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNipbl (Nipbl Mouse)
ExpressionMammalianAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-2xPH-TAPP1 R211L mutant
Plasmid#161991PurposeExpresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPH-TAPP1 (PLEKHA1 Human)
ExpressionMammalianMutationR211LAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
RKS-Fzd8 subtype NGS Wnt
Plasmid#159627PurposeExpress Fzd8 subtype NGS Wnt in mammalian cellsDepositorInsertFzd8 subtype NGS Wnt
UseLentiviralTagsHis tagExpressionMammalianAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only