174,421 results

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BMP4_pLX307

Plasmid

#98321

Purpose

Lentiviral expression of BMP4

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BMP4 (BMP4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pProEx-IDE-wt

Plasmid

#99014

Purpose

Express human Insulin Degrading Enzyme (IDE) in E. coli.

Depositor

Wei-Jen Tang

Article

Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

Insert

insulin degrading enzyme (IDE Human)

Tags

His tag

Expression

Bacterial

Promoter

TRC

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-BRCA1

Plasmid

#99394

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1

Depositor

Junjie Chen, Michael Huen

Article

Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

BRCA1 (BRCA1 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry

Plasmid

#85430

Purpose

[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-10 T2A PD-L1

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

Plasmid

#90086

Purpose

This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA

Depositor

Raffaella Sordella

Article

Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

Destabilized Domain and Flag

Expression

Mammalian

Promoter

EFS

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0501

Plasmid

#91011

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-human Igf2bp2-IRES-GFP

Plasmid

#91890

Purpose

Retroviral expression of human Igfbp2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Igf2bp2 (IGF2BP2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV

Plasmid

#91719

Purpose

AAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2

Depositor

Ryohei Yasuda

Article

Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

Insert

mEGFP-P2A-paAIP2(R5AR6A)

Use

AAV

Promoter

CaMKII 1.3

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-GalT

Plasmid

#87327

Purpose

To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

Depositor

Lei Lu

Article

Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

Insert

1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-mtagBFP

Plasmid

#89685

Purpose

pCAG promoter driving mtagBFP

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

Plasmid

#87694

Purpose

Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

ERT2-PhoCl-Cre-PhoCl-ERT2

Expression

Mammalian

Promoter

Chicken beta actin

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_WT

Plasmid

#81734

Purpose

Gateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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1179_pAAV-U6-BbsI-gRNA-CB-EmGFP

Plasmid

#89060

Purpose

AAV-gRNA cloning vector with GFP reporter

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK1tight

Plasmid

#89362

Purpose

A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1tight (MAP2K1 Human, Synthetic)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET32bplus_trx_dAChE4

Plasmid

#83917

Purpose

Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli

Depositor

Sarel Fleishman

Article

Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

Insert

Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)

Tags

His, S-tag, and TRX

Expression

Bacterial

Mutation

V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…

Promoter

T7 promoter

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-mTubb3

Plasmid

#87116

Purpose

AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA

Use

AAV and CRISPR

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3_V5-APEX2-Sec61B

Plasmid

#83412

Purpose

Expressed N-term tagged APEX2 on Sec61B in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-Sec61B (SEC61B Human, Synthetic)

Tags

APEX2 and V5

Expression

Mammalian

Promoter

CMV-F

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT G250A

Plasmid

#84925

Purpose

luciferase reporter for mutant TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

G250A

Promoter

TERT

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
pHAGE2 GFP-Rtn4a

Plasmid

#86684

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4a (RTN4 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

Plasmid

#86885

Purpose

C-terminal part of the split-BioID

Depositor

Mathieu Bollen

Article

Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

Insert

BirA* (AA141-321)- HA- NIPP1]

Tags

HA tag

Expression

Mammalian

Mutation

BirA Amino Acids 141-321

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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