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MXS_MCS2

Plasmid

#62396

Purpose

MXS_chaining vector with MCS2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJK_proK6_eGFP

Plasmid

#59850

Purpose

Constitutively expresses eGFP with proK6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK6

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJB073

Plasmid

#98912

Purpose

Express Dronpa tagged ZapB in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

ZapB

Tags

Dronpa

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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FUGW-H1-Syndecan-1 shRNA (UTR)

Plasmid

#40623

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Syndecan-1 shRNA(UTR) (Sdc1 Mouse)

Use

Lentiviral and RNAi

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pTK_Snai2_enh_332_Citrine

Plasmid

#130577

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Snai2_enh_332

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Snai2_enh_242_Citrine

Plasmid

#130576

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Snai2_enh_242

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Snai2_enh_239_Citrine

Plasmid

#130575

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Snai2_enh_239

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Snai2_enh_334_Citrine

Plasmid

#130578

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Snai2_enh_334

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-L3S1P51_T

Plasmid

#127037

Purpose

Basic Part T- Terminator; L3S1P51; synthetic terminator

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P51_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pbad_P

Plasmid

#127003

Purpose

Basic Part P- Promoter (non-constitutive); Pbad (I13453), arabinose induced; controlled by AraC.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pbad

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-T7pro_P

Plasmid

#127002

Purpose

Basic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymerase

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part T7 promoter_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b- hTPI Ile170Val

Plasmid

#50724

Purpose

overexpression of the human TPI1 Ile170Val allele in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Tags

6xHIS tag

Expression

Bacterial

Mutation

changed Isoleucine 170 to Valine

Promoter

T7

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Streptag_N

Plasmid

#127034

Purpose

Basic Part N- N-terminus; Strep-tag II

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part StrepII_tag_N

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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miR-vector plix4.03

Plasmid

#97141

Purpose

For lentiviral tet-inducible expression of miRNA and Dendra2

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Type

Empty backbone

Use

Lentiviral

Tags

dendra2

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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AIRAP 4: AIRAP.GST.V3.pCDNA3

Plasmid

#21794

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Tags

GST

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pTK_TFAP2b_enh_338_Citrine

Plasmid

#130619

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

TFAP2b_enh_338

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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RALB

Plasmid

#55658

Purpose

expression of mouse RALB

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RALB (Ralb Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pAP639-2F

Plasmid

#99486

Purpose

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-24

Plasmid

#120003

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-10

Plasmid

#119989

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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