We narrowed to 12,866 results for: sequence

1. pCIneo-lambdaN-HA-HsAGO2-F2V2_F

   Plasmid

   #146287

   Purpose

   Mammalian Expression of HsAGO2-F2V2

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   HsAGO2-F2V2 (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCIneo-lambdaN-HA-HsAGO2-F470V_F

   Plasmid

   #146288

   Purpose

   Mammalian Expression of HsAGO2-F470V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   HsAGO2-F470V (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIneo-lambdaN-HA-HsAGO2-F505V_F

   Plasmid

   #146289

   Purpose

   Mammalian Expression of HsAGO2-F505V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   HsAGO2-F505V (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-lambdaN-HA-HsAGO2-F470V_E

   Plasmid

   #146225

   Purpose

   Insect Expression of HsAGO2-F470V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   HsAGO2-F470V (AGO2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-HsAGO2-F505V_E

   Plasmid

   #146226

   Purpose

   Insect Expression of HsAGO2-F505V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   HsAGO2-F505V (AGO2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLY227

   Plasmid

   #184148

   Purpose

   short crRNA generator with spacer LEA2 with WT repeat region

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   s-crRNA-LEA2-WT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Plux2

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENT-L3-pSnail-L5

   Plasmid

   #186365

   Purpose

   Entry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequences

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Snail regulatory sequence
   

   Use

   Protein expression

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLY232

   Plasmid

   #184149

   Purpose

   short tracrRNA generator with WT anti-repeat region

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   s-tracrRNA-WT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLY171

   Plasmid

   #184145

   Purpose

   sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   sgRNA-LEA2-ex3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Plux2

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLY283

    Plasmid

    #184156

    Purpose

    Reprogrammed short tracrRNA for hijacking mRNA of zntA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-zntA-1034
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENT-L3-pFOGl-L5

    Plasmid

    #186364

    Purpose

    Entry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Friend Of Gata regulatory sequence
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPR2_+10D+7U

    Plasmid

    #149393

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    7bp inserted upstream, and 10bp inserted downstre…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR2_+10D+10U

    Plasmid

    #149394

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    10bp inserted upstream and downstream of the IHFp…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPR2_IHF_Mut

    Plasmid

    #149396

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHFprox site is mutated at key positions needed f…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPR2_motif_scram

    Plasmid

    #149397

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Upstream motif recognized by Cas1-2/3 during spac…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid

    #179528

    Purpose

    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 R1333A/R1335A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginines 1333 and 1335 to alanine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJCC_103 SpCas9 T1337C

    Plasmid

    #179525

    Purpose

    For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 T1337C
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed threonine 1337 to cysteine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFX-H2B-Sirius

    Plasmid

    #175837

    Purpose

    Express Sirius with nucleus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with nucleus-targeting sequence
    

    Use

    Tags

    H2B (human histone 2B)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFX-iRFP-Rab5

    Plasmid

    #174456

    Purpose

    Express iRFP with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with early endosome-targeting sequence
    

    Use

    Tags

    small GTPase (Rab5)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFX-SECFP-Sec61β

    Plasmid

    #174465

    Purpose

    Express SECFP with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    SECFP with endoplasmic reticulum-targeting sequence
    

    Use

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFX-iRFP-KRasCT

    Plasmid

    #175836

    Purpose

    Express iRFP with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with plasma membrane-targeting sequence
    

    Use

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFX-Sirius-Sec61β

    Plasmid

    #174464

    Purpose

    Express Sirius with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with endoplasmic reticulum-targeting sequence
    

    Use

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFX-GalT-Sirius

    Plasmid

    #174469

    Purpose

    Express Sirius with golgi apparatus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with golgi apparatus-targeting sequence
    

    Use

    Tags

    β1,4-galactose transferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPLN-hFluc-WT-KDEL

    Plasmid

    #177715

    Purpose

    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    KDEL and Prolactin Signal Sequence

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NmMetQ

    Plasmid

    #172111

    Purpose

    Expression of Methionine Binding Protein (MetQ) with signal sequence

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Insert

    Methionine Binding Protein
    

    Use

    Tags

    10x Histidine Tag and N.meningitidis signal seque…

    Expression

    Bacterial

    Mutation

    Changed Proline 22 to Alanine

    Promoter

    T7 Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBS-V7

    Plasmid

    #173796

    Purpose

    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene latifolia clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation

    Depositor

    Daniel Sloan

    Article

    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.

    Inserts

    aadA
    ClpP1
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pBS-V6

    Plasmid

    #173795

    Purpose

    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene conica clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation

    Depositor

    Daniel Sloan

    Article

    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.

    Inserts

    aadA
    ClpP1
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pBS-V4

    Plasmid

    #173794

    Purpose

    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Nicotiana tabacum clpP1 gene with native regulatory elements and flanking regions for tobacco plastome transformation

    Depositor

    Daniel Sloan

    Article

    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.

    Inserts

    aadA
    ClpP1
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pAV-U6+27Linear-1, PolyU(0), Hairpin Distance = 0nt

    Plasmid

    #166993

    Purpose

    Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(0), Hairpin = 0 nt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt

    Plasmid

    #166986

    Purpose

    Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(1), Hairpin = 0 nt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge