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We narrowed to 10,790 results for: T7

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Showing: 2321 - 2360 of 10790 results

  3. pJK122

    Plasmid
    #74295
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusion
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Tags
    NusA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74295-plasmid-map-sequence-id-141881

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  4. pJK237

    Plasmid
    #73339
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Tags
    His6, Ssp intein, and chitin-binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73339-plasmid-map-sequence-id-138207

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  5. pHT-PRD

    Plasmid
    #87755
    Purpose
    Bacterial expression vector for producing 6x histidine tagged dynamin 1a proline rich domain (residues 746-864)
    Depositor
    Yu-Wen Hwang
    Insert
    Semi-synthetic gene coding for dynamin 1a proline rich domain (residues 746-864)
    Tags
    6X histidine tag
    Expression
    Bacterial
    Promoter
    T7 RNA polymerase promoter
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87755-plasmid-map-sequence-id-173677

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  6. pT7-7 asyn WT1-106 Mxe

    Plasmid
    #105746
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein 1-106 Mxe (SNCA Human)
    Expression
    Bacterial
    Mutation
    1-106AA Mxe
    Promoter
    T7
    Available Since
    May 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105746-plasmid-map-sequence-id-209704

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  7. pT7-7 asyn K32R/K34R

    Plasmid
    #105741
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein K32R/K34R (SNCA Human)
    Expression
    Bacterial
    Mutation
    K32R/K34R
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105741-plasmid-map-sequence-id-265018

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  8. pET11a asyn Q79N (Q109H) (TAC-->TAT)

    Plasmid
    #105736
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein Q79N (Q109H) (TAC-->TAT) (SNCA Human)
    Expression
    Bacterial
    Mutation
    Q79N/Q109H
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105736-plasmid-map-sequence-id-207269

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  9. pAJC1

    Plasmid
    #240164
    Purpose
    Production of a thermostable enzyme, Pyrobaculum calidifontis manganese-dependent catalase, to be used in an educational laboratory exercise.
    Depositor
    Danielle Saunders
    Inserts
    katPc
    Pc- kat
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    240164-plasmid-map-sequence-id-478436

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  10. pET15bHis6-pG-Tnp

    Plasmid
    #124884
    Purpose
    To transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.
    Depositor
    Keji Zhao
    Insert
    protein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    124884-plasmid-map-sequence-id-243752

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  11. pET-Impact-T4gp32(L301G)

    Plasmid
    #228209
    Purpose
    Expression in BL21(DE3) of single strand DNA binding protein L301G
    Depositor
    Stephen Benkovic
    Insert
    61
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated leucine 301 to glycine (L301G), improves …
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228209-plasmid-map-sequence-id-453946

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  12. pAIP4_4QMD

    Plasmid
    #234174
    Purpose
    Heterologous protein expression of envoplakin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4QMD (EVPL Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234174-plasmid-map-sequence-id-464139

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  13. pAIP4_1BKR

    Plasmid
    #234168
    Purpose
    Heterologous protein expression of spectrin beta chain, non-erythrocytic 1 (Beta-II spectrin) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1BKR (SPTBN1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234168-plasmid-map-sequence-id-464134

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  14. pAIP4_2JIK

    Plasmid
    #234170
    Purpose
    Heterologous protein expression of synaptojanin-2-binding protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2JIK (SYNJ2BP Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234170-plasmid-map-sequence-id-464136

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  15. pAIP4_5EF9

    Plasmid
    #234154
    Purpose
    Heterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5EF9 (PB2 Influenza B virus (B/Lee/1940))
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234154-plasmid-map-sequence-id-464120

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  16. pT7CFE1_EVA71_VP12A_C110A

    Plasmid
    #212976
    Purpose
    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A C110A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Mutation
    changed cysteine 110 from 2A to alanine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212976-plasmid-map-sequence-id-420360

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  17. RFXANK:JMC029-A02:C213521

    Plasmid
    #183980
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    RFXANK (RFXANK Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183980-plasmid-map-sequence-id-361429

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  18. MPP1:HPC09T-D06:C210522

    Plasmid
    #183976
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    MPP1 (MPP1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183976-plasmid-map-sequence-id-361413

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  19. pmCellFree_KA1_SARS-COV-2_ORF7b

    Plasmid
    #169394
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169394-plasmid-map-sequence-id-336882

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  20. pSBbi-Pur-AktAR2-D

    Plasmid
    #125196
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2-D
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125196-plasmid-map-sequence-id-243601

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  26. pET11a asyn Q79N Q109N (TAC-->TAT)

    Plasmid
    #105738
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
    Expression
    Bacterial
    Mutation
    Q79N/Q109N
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105738-plasmid-map-sequence-id-207283

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  27. 2QNR (NEDD5)

    Plasmid
    #101273
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NEDD5 (SEPTIN2 Human)
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101273-plasmid-map-sequence-id-193507

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  28. pJK103

    Plasmid
    #73265
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73265-plasmid-map-sequence-id-134809

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  29. KCTD17

    Plasmid
    #74661
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KCTD17A (KCTD17 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  30. pJK610

    Plasmid
    #72531
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72531-plasmid-map-sequence-id-132619

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  31. pJK540

    Plasmid
    #72446
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72446-plasmid-map-sequence-id-133865

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  32. pJK552

    Plasmid
    #72451
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Val319 to Phe and inserted Val2
    Promoter
    T7
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72451-plasmid-map-sequence-id-133883

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  33. pJK586

    Plasmid
    #72459
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72459-plasmid-map-sequence-id-133898

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  34. pJK565

    Plasmid
    #72454
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Ala/Trp165>Phe mutant (AaPurE1fwf-H59A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72454-plasmid-map-sequence-id-133894

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  35. pJK578

    Plasmid
    #72457
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Ala/Trp165>Phe mutant (AaPurE1fwf-S57A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72457-plasmid-map-sequence-id-133895

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  36. pJK569

    Plasmid
    #72455
    Purpose
    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~51 silent substitutions
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72455-plasmid-map-sequence-id-133906

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  37. pJK585

    Plasmid
    #72458
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Cys/Trp165>Phe mutant (AaPurE1fwf-S57C)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72458-plasmid-map-sequence-id-133896

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  38. pJK525

    Plasmid
    #71601
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tryptopha…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71601-plasmid-map-sequence-id-131675

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  39. pJK526

    Plasmid
    #71602
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-97 to tyrosine, tryptophan-165…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71602-plasmid-map-sequence-id-131676

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  40. pJK602

    Plasmid
    #71607
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe mutant (AaPurE1-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tyrosine-154 …
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71607-plasmid-map-sequence-id-131684

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Showing: 2321 - 2360 of 10790 results