Addgene

We narrowed to 12,997 results for: SHI

Showing: 2901 - 2950 of 12997 results

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pT7-L1T3D

Plasmid

#33276

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

L1 (L1, L1 Orthoreovirus T3D)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-Klf4-IP

Plasmid

#15920

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Kruppel-like factor 4 (gut) (Klf4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pENTR1A-SynTetOff-GFP

Plasmid

#190230

Purpose

Gateway entry vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV2-DEST(f)

Plasmid

#190226

Purpose

Gateway destination vector, empty AAV vector backbone

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET30 SUMO-1 G97

Plasmid

#13381

Depositor

Mary Dasso

Article

Saitoh et al Curr Biol. 1998 Jan 15. 8(2):121-4.

Insert

SUMO-1, G97 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

G97 mutant: lacks 4 amino acids from the C-termin…

Promoter

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pHDAdVenus-geo-TK

Plasmid

#26423

Depositor

Kohnosuke Mitani

Article

Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

PGK-b-geo, HSV-TK, CAG-Venus

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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pMALp2x-CARP-1

Plasmid

#41727

Depositor

Koji Okamoto

Article

Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

clam ADP-ribosylating protein-1

Use

Bacterial subcloning

Tags

Expression

Mutation

deleted 5'-UTR nucleotides 1-64

Promoter

pMB1

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pET28b_RanWT

Plasmid

#186270

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Use

Tags

His tagged

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV Puro SCRIB WT

Plasmid

#88886

Purpose

SCRIB WT in MSCV backbone

Depositor

Senthil Muthuswamy

Article

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Tags

N/A

Expression

Mutation

N/A

Promoter

MSCV promoter

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pIZ-Flag6His-Siwi

Plasmid

#50560

Purpose

Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Article

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

Siwi (Aubergine )

Use

Tags

6xHIS and FLAG

Expression

Insect

Mutation

Promoter

OpIE2

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV2-SYN-GFP-BGHpA

Plasmid

#190229

Purpose

Expresses EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pT2ADW-lox-mCherry-hyBRET-ERK-NLS

Plasmid

#170290

Purpose

For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

lox-mCherry-lox-hyBRET-ERK-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfvAChT-1.8k-Venus-WPRE

Plasmid

#156389

Purpose

Expresses Venus under vAChT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-plasma membrane/pcDNA3

Plasmid

#51975

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

HIS and Lyn

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pTYF-super-mfSST-0.3k-Venus-WPRE

Plasmid

#156393

Purpose

Expresses Venus under SST gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CRY2-mCherry

Plasmid

#178525

Purpose

Expresses CRY2-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CRY2-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-ERas-IP

Plasmid

#13826

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pNIC28-Bsa4-ICL1

Plasmid

#105822

Purpose

Expression of Mycobacterium tuberculosis icl1 recombinant protein

Depositor

Ivanhoe Leung

Article

Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

Insert

icl1

Use

Tags

His tag with TEV cleavage

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPV-0.8k-Venus-WPRE

Plasmid

#156399

Purpose

Expresses Venus under PV gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-super-mfPENK-0.9k-Venus-WPRE

Plasmid

#156401

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-SPW

Plasmid

#190628

Purpose

Bacterial expression of genetically encoded protease indicator SPW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SPW

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)

Plasmid

#191177

Purpose

Mammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP (frameshifted)

Use

Tags

Expression

Mammalian

Mutation

EGFP is frameshifted

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA-SCW

Plasmid

#190633

Purpose

Mammalian expression of genetically encoded calcium indicator SCW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-SKW

Plasmid

#190631

Purpose

Bacterial expression of genetically encoded potassium indicator SKW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SKW

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-SCW

Plasmid

#190626

Purpose

Bacterial expression of genetically encoded calcium indicator SCW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Nano-lantern-H2B/pcDNA3

Plasmid

#51971

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

human H2B

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pTYF-super-mfPENK-2.2k-Venus-WPRE

Plasmid

#156400

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-EGFP-Rheb-HVR HRas-IP

Plasmid

#13833

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb-HVR HRas (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminal of Rheb and hyper variable region (HVR…

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pME18S-3HA-mULK2(K39T)

Plasmid

#22899

Depositor

Masaaki Muramatsu

Article

Yan et al Oncogene. 1999 Oct 21. 18(43):5850-9.

Insert

UNC-51-like kinase 2 (Ulk2 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

changed Lysine 39 to Threonine

Promoter

Available since

Sept. 14, 2010

Availability

Academic Institutions and Nonprofits only
pTYF-super-mfSERT-1.9k-Venus-WPRE

Plasmid

#156394

Purpose

Expresses Venus under SERT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAE-MIBP1

Plasmid

#51873

Purpose

Expresses full length rat MIBP1 (cloned in pCAGGS)

Depositor

Kenshi Hayashi, Tomoko Tahira

Article

Fukuda et al J Biochem. 2002 Mar;131(3):349-57.

Insert

MIBP1 (Hivep2 Rat)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pMath3-luc

Plasmid

#51312

Purpose

Luciferase reporter driven by the Math3 promoter

Depositor

Ryoichiro Kageyama

Article

Tsuda et al J Biol Chem. 1998 Mar 13;273(11):6327-33

Insert

Math3 promoter (-2.8k- +196) (Neurod4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
pX330_NPM2_cterm1

Plasmid

#222911

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 1 (NPM2 Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_NPM2_cterm4

Plasmid

#222914

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 4 (NPM2 Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_NPM2_cterm3

Plasmid

#222913

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 3 (NPM2 Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_NPM2_cterm2

Plasmid

#222912

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 2 (NPM2 Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-TdIF2

Plasmid

#196906

Purpose

visualization of TdIF2/ERBP as a fusion to mCherry

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

TdIF2

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-TdIF2

Plasmid

#199699

Purpose

visualization of nucleolus in living mammalian cells

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

TdIF2

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTC3cIfm

Plasmid

#193778

Purpose

Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Frame-shifted CI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLTetO-1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
etnppl-pGEMtEasy

Plasmid

#192263

Purpose

Mouse Etnppl sequence is inserted in pGEM-T Easy vector.

Depositor

Toshihide Yamashita

Article

Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

Insert

ethanolamine phosphate phospholyase (Etnppl Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-15b(ETNPPL)

Plasmid

#192264

Purpose

Coding sequence of mouse ETNPPL is inserted in pET-15b vector

Depositor

Toshihide Yamashita

Article

Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

Insert

ethanolamine phosphate phospholyase (Etnppl Mouse)

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-RCC

Plasmid

#190635

Purpose

Mammalian expression of genetically encoded calcium indicator RCC

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

RCC

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-DEST(r)

Plasmid

#190238

Purpose

Gateway destination vector, empty AAV vector backbone

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-SCcpV

Plasmid

#190636

Purpose

Mammalian expression of genetically encoded calcium indicator SCcpV

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCcpV

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
YF

Plasmid

#171459

Purpose

Expresses YF, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

EYFP-FKBP12

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST-Fah_S

Plasmid

#163088

Purpose

expressing mouse swingshift mutant FAH in bacterial cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Insert

Fah_S

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Fah_S

Plasmid

#163086

Purpose

Swingshift mutant FAH in mammalian cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Inserts

Fah_S
Fah_S

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-super-mfPV-1.8k-Venus-WPRE

Plasmid

#156398

Purpose

Expresses Venus under PV gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-super-mfSP-0.8k-Venus-WPRE

Plasmid

#156397

Purpose

Expresses Venus under SP gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-super-mfSP-1.7k-Venus-WPRE

Plasmid

#156396

Purpose

Expresses Venus under SP gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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