We narrowed to 22,359 results for: ESC;

1. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

   Plasmid

   #216733

   Purpose

   Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase and sgCTG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CAG

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX551-miniCMV-SpCas9D10A nickase

   Plasmid

   #216735

   Purpose

   Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   T7 and mini-CMV

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-U6-AlbEx14-mNG

   Plasmid

   #201780

   Purpose

   knocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in mice

   Depositor

   Hajime Hirase

   Article

   Vittani et al Fluorescence Imaging of the Brain. Neuro

   Inserts

   Alb gRNA (Alb Mouse)
   Alb_LHA-mNeonGreen-Alb_RHA (Alb Mouse)
   

   Use

   AAV and CRISPR

   Promoter

   U6

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FLAG-RPS14(89-151)

   Plasmid

   #122299

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1 to 88

   Promoter

   CMV

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-RPS14(2-88)

   Plasmid

   #122298

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (partial) (RPS14 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 89 to 151

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF1522

   Plasmid

   #143288

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR2E3 (NR2E3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF1521

   Plasmid

   #144181

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR2E3 (NR2E3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-BBclone1

   Plasmid

   #137069

   Purpose

   E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Inserts

   BB0405 (BB_0405 Borrelia burgdorferi B31)
   BB0406
   P66
   BBA57
   HtrA
   RevA (revA Borrelia burgdorferi B31)
   BB0238
   

   Expression

   Bacterial

   Mutation

   wild-type, full-length (signal-peptide-containing…

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-EGFP

   Plasmid

   #13031

   Purpose

   Mammalian expression vector for cloning your gene fused to EGFP

   Depositor

   Doug Golenbock

   Insert

   Enhanced Green Fluorescent Protein
   

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-mLemon

    Plasmid

    #191256

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein mLemon

    Depositor

    Robert Campbell

    Insert

    mLemon
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAIP4_5M2Y

    Plasmid

    #234193

    Purpose

    Heterologous protein expression of B7LG64_ECO55 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5M2Y
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLenti6.2_miRFP670

    Plasmid

    #113726

    Purpose

    Expresses the fluorescent protein miRFP670 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    miRFP670
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD-pHmGL

    Plasmid

    #191257

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein pHmGL

    Depositor

    Robert Campbell

    Insert

    pHmGL
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK515

    Plasmid

    #72385

    Purpose

    Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylglycinamide formyltransferase 2
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMflT-o4-mNG

    Plasmid

    #201855

    Purpose

    Mesoplasma florum oriC plasmid expressing mNeonGreen fluorescent protein

    Depositor

    Sébastien Rodrigue

    Insert

    mNeonGreen
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMflT-o4-YFP

    Plasmid

    #201856

    Purpose

    Mesoplasma florum oriC plasmid expressing LanYFP fluorescent protein

    Depositor

    Sébastien Rodrigue

    Insert

    LanYFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-CFP

    Plasmid

    #13030

    Purpose

    Mammalian expression vector for cloning your gene fused to CFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.2_mTurquoise2

    Plasmid

    #113723

    Purpose

    Expresses the fluorescent protein mTurquoise2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-pHScarlet

    Plasmid

    #191258

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet

    Depositor

    Robert Campbell

    Insert

    pHScarlet
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.2_TagBFP

    Plasmid

    #113724

    Purpose

    Expresses the fluorescent protein TagBFP in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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