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C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r

Plasmid

#162936

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Triple FLAG epitope tag fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E01: attB5r-meGFP-nostop-attB1r

Plasmid

#162940

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric enhanced Green fluorescent protein

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

Plasmid

#162937

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E11: attB5r-RU5u-His6-nostop-attB1r

Plasmid

#162953

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E25: attB4-mPol2p>-attB5

Plasmid

#162981

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the murine RNA polymerase 2 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

murine RNA polymerase 2 promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r

Plasmid

#162956

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r

Plasmid

#162935

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Maltose-binding protein fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E09: attB5r-RU5u-FLAG-nostop-attB1r

Plasmid

#162951

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

FLAG epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E59: attB5r-RU5u-2xHA-nostop-attB1r

Plasmid

#162966

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual HA epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E42: attB5r-RU5u-Strep2-nostop-attB1r

Plasmid

#162961

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E26: attB5r-RU5u-myc-nostop-attB1r

Plasmid

#162960

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Myc epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E10: attB5r-RU5u-HA-nostop-attB1r

Plasmid

#162952

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal HA-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

HA epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E10: attB4-FerHp>-attB1r

Plasmid

#162969

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ferritin heavy chain promoter

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E24: attB5r-RU5u-V5-nostop-attB1r

Plasmid

#162959

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal V5-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

V5 epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E34: attB5r-iRFP670-nostop-attB1r

Plasmid

#162947

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

far-red iRFP670 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

Plasmid

#162932

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion with AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C415-E01: attB4-stuffer-attB1r

Plasmid

#162931

Purpose

Gateway attB4-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-R251

Plasmid

#134307

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 R251A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-S245

Plasmid

#134302

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3_zfh1_CP-candidate_luc+

Plasmid

#86391

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer

Use

Luciferase

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only