We narrowed to 9,182 results for: Pol;

1. 3TERM_MpNOP1

   Plasmid

   #136119

   Purpose

   3TERM after CDS/ CDS12-CTAG

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   3TERM_MpNOP1 Mapoly0008s0036
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. 5UTR_MpNOP1

   Plasmid

   #136103

   Purpose

   5UTR for cloning with PROM_MpNOP1

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   5UTR_MpNOP1 Mapoly0008s0036
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. CDS12_TP-MpSIG2

   Plasmid

   #136093

   Purpose

   Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   TP-MpSIG2, Mapoly0214s0004
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. SZ_EC_02

   Plasmid

   #155341

   Purpose

   Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR

   Depositor

   Jens Nielsen

   Article

   Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:

   Inserts

   tef1 promoter - xylulose kinase - pex20 terminator
   pyk1 promoter - xylose reductase - pex16 terminator
   gapdh promoter - xylitol dehydrogenase - lip2 terminator
   

   Use

   Easycloneyali insert vector

   Expression

   Yeast

   Promoter

   gapdh, pyk1, and tef1

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVSL10

   Plasmid

   #201182

   Purpose

   Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable

   Depositor

   Jack Szostak

   Article

   Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.

   Insert

   Bst polymerase large fragment (BF) F710Y/D598A
   

   Tags

   HRV 3C protease cleavage site and His6

   Expression

   Bacterial

   Mutation

   F710Y D598A

   Promoter

   T5-lacO

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA6 MCV LTco S220A

   Plasmid

   #112193

   Purpose

   Expression of MCPyV LT mutant (Ser 220 to Ala)

   Depositor

   Patrick Moore

   Article

   Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

   Insert

   MCPyV LT codon optimized
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. E3020X

   Plasmid

   #21366

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Expression

   Mammalian

   Mutation

   Changed E3020 to stop codon

   Available Since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

8. WT (REP10)

   Plasmid

   #21368

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. WT (AF20)

   Plasmid

   #21359

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   wild-type sequence*

   Available Since

   Jan. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. PROM_MpUBE2

    Plasmid

    #136109

    Purpose

    PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_MpUBE2 Mapoly0106s0019
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. 5UTR_MpUBE2

    Plasmid

    #136110

    Purpose

    5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    5UTR_MpUBE2 Mapoly0106s0019
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA6 MCV LTco S239A

    Plasmid

    #112194

    Purpose

    Expression of MCPyV LT mutant (Ser 239 to Ala)

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCPyV LT codon optimized
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM41-EcLonA

    Plasmid

    #38330

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    Lon protease fragment (lon Escherichia coli)
    

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    Polyphosphate binding fragment of Lon protease. …

    Promoter

    T7

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA6 MCV LTco S147A

    Plasmid

    #112192

    Purpose

    Expression of MCPyV LT mutant (Ser 147 to Ala)

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCPyV LT codon optimized
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MCV-HF LTS220A/S239A

    Plasmid

    #112191

    Purpose

    Merkel cell polyomavirus molecular clone

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCV-HF LTS220A/S239A
    

    Mutation

    T to G mutations at 3313 (nt) and 3370 (nt) of fu…

    Promoter

    None from vector. Insert contains native promoter…

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGO186N-KS1

    Plasmid

    #174301

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Expression

    Bacterial

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGO186N-KS2

    Plasmid

    #174302

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Expression

    Bacterial

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGO186N-KS3

    Plasmid

    #174303

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PROM5_MpU6

    Plasmid

    #136120

    Purpose

    Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM5_MpU6
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CDS12-MpER-Targ

    Plasmid

    #136094

    Purpose

    CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    ER targeting peptide from predicted chitinase Mapoly0069s0092
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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