We narrowed to 5,545 results for: SPI

1. p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)

   Plasmid

   #224537

   Purpose

   A Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-(n2)StayGold(c4)-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. plentiCRISPR-v2 Puro SPNS1_sg2

   Plasmid

   #218531

   Purpose

   sgRNA targeting human SPNS1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SPNS1 (SPNS1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. plentiCRISPR-v2 Puro SPNS1_sg1

   Plasmid

   #218530

   Purpose

   sgRNA targeting human SPNS1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SPNS1 (SPNS1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.11_Nued2_E5

   Plasmid

   #203834

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 5

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   E5 (Igf1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24a-LaM4-2xTS

   Plasmid

   #162779

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE

   Plasmid

   #203847

   Purpose

   Flp-dependent expression of Chronos fused to EGFP

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   Chronos
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-3x-FLAG-Ubiquitin (JDW 1098)

   Plasmid

   #224513

   Purpose

   A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3x-FLAG-Ubiquitin
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

   Plasmid

   #104563

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1 (D832A)
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-DreERT2 (JDW 1344)

   Plasmid

   #224523

   Purpose

   A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   DreERT2
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

    Plasmid

    #224490

    Purpose

    Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Golgi-mTagBFP-P2A-H2A-iRFP
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME Actin Vhh mCherry (JDW 1240)

    Plasmid

    #224499

    Purpose

    Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mCherry
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGEN-WPRE-bGH-DEST (JDW 1309)

    Plasmid

    #229815

    Purpose

    A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

    Plasmid

    #229828

    Purpose

    A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p5E-dr-myl7-pro (JDW 1148)

    Plasmid

    #229836

    Purpose

    A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    cmlc2/myl7
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pME-3xNLS-mTagBFP2-Cre (JDW 918)

    Plasmid

    #224517

    Purpose

    A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xNLS-mTagBFP2-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME Actin-Vhh sfGFP (JDW 1215)

    Plasmid

    #224495

    Purpose

    Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-sfGFP
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME-mScarlet3-Giantin (JDW 1356)

    Plasmid

    #224502

    Purpose

    Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet3-Giantin
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pME-EGFP-P2A-iCre (JDW 921)

    Plasmid

    #224519

    Purpose

    A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EGFP-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME EFS-rtTA/rtTA3 (JDW 1230)

    Plasmid

    #224525

    Purpose

    A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EFS-rtTA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAdTrack-CMV HA ALAS-1

    Plasmid

    #22746

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    ALAS-1 (Alas1 Mouse)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only