We narrowed to 5,441 results for: SPI

1. pT7-7 asyn A30P

   Plasmid

   #105726

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

   Insert

   alpha-synuclein A30P (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A30P

   Promoter

   T7

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-PGC1(1-400)

   Plasmid

   #1029

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains only aa1-400

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-Ef1a-fDIO-mRuby3-2A-dCre

   Plasmid

   #203845

   Purpose

   Flp-dependent enzymatically inactive Cre and mRuby3 expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dCre
   

   Use

   AAV

   Tags

   mRuby3-2A

   Expression

   Mutation

   Enzymatically inactive Cre

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-3xNLS-(G2SG2)2-Cre (JDW 1149)

   Plasmid

   #224514

   Purpose

   A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   3xNLS-(G2SG2)2-Cre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E-TetO8x-CMVmin (JDW 1087)

   Plasmid

   #224475

   Purpose

   Gateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   TetO(8x)-CMV promoter
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-Flex-mRuby3-2A

   Plasmid

   #203846

   Purpose

   Neuron specific expression of a Cre-dependent mRuby3

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RBD-Fc

   Plasmid

   #164221

   Purpose

   Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

   Insert

   Spike RBD (S SARS-CoV)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI

   Plasmid

   #192112

   Purpose

   Expresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Insert

   SARS-CoV-2-S2P-BA.4/5 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET24a-LaM4-3xTS

   Plasmid

   #162777

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET24a-VHH-myc

    Plasmid

    #109422

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-PRDM16 880-1038

    Plasmid

    #53350

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 880-1038

    Promoter

    tac

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. GST-PRDM16 224-454

    Plasmid

    #53347

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 224-454

    Promoter

    tac

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCMX-PGC1b

    Plasmid

    #1032

    Depositor

    Bruce Spiegelman

    Article

    Lin et al J Biol Chem 2002 Jan 18;277(3):1645-8. E

    Insert

    PGC-1 beta (Ppargc1b Mouse)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

14. GST-PRDM16 1-223

    Plasmid

    #53346

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 1-223

    Promoter

    tac

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. Gal4 PGC1 alpha 1-550

    Plasmid

    #8894

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Gal4 DBD

    Expression

    Mammalian

    Mutation

    amino acids 1-550 of PGC-1 alpha

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

16. GST-PRDM16 1039-1176

    Plasmid

    #53351

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 1039-1176

    Promoter

    tac

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. GST-PRDM16 681-880

    Plasmid

    #53349

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 681-880

    Promoter

    tac

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. GST-PRDM16 455-680

    Plasmid

    #53348

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 455-680

    Promoter

    tac

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. ANKRD24-TwinStrepII

    Plasmid

    #83190

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Article

    Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

    Insert

    ANKRD24 (Ankrd24 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET24a-VHH-tev-APEX2

    Plasmid

    #117751

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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