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pLKO.1-Teton-puro-shFAK #2

Plasmid

#198758

Purpose

conditional knockdown of FAK

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

Insert

shFAK #2 (PTK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Tet1-Poly(A)

Plasmid

#65559

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-SV40P-Tet2 enhancer H1

Plasmid

#63883

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 enhancer fragment H1

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)

Plasmid

#70212

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

tetR

Use

Tags

Expression

Mutation

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro shPOLE1_1

Plasmid

#160810

Purpose

Express Dox repressible shPOLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pTetO-Kir2.1-IRES-TLZ

Plasmid

#32642

Depositor

Richard Axel, Joseph Gogos, C. Ron Yu

Article

Yu et al Neuron. 2004 May 27;42(4):553-66.

Insert

tetO-Kir2.1-IRES-TLZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GIN-recipient

Plasmid

#11656

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP, IRES, and Neo

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shID2 #1

Plasmid

#83086

Purpose

Lentiviral shRNA vector for inducible knockdown of human ID2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shID2 (ID2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pGL3-SV40P-Tet1 enhancer E

Plasmid

#63880

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 enhancer fragment E

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTC-CMV-Tet-YAP

Plasmid

#85579

Purpose

Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (CMV promoter ) in mouse liver

Depositor

Ursula Ehmer

Article

Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

Inserts

CreER
YAP

Use

Cre/Lox

Tags

Flag (2x)

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pTNT-pT7-tetR-sfGFP

Plasmid

#140868

Purpose

T7 promoter driven synthesis of C-terminal fusion of sfGFP to TetR repressor (TetR-sfGFP)

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-sfGFP expressed with T7 promoter

Use

Synthetic Biology

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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PRS415-Gal1pr-sctetR-FokI

Plasmid

#44756

Depositor

Narendra Maheshri

Article

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

sctetR-FokI (fokI Synthetic)

Use

Tags

Expression

Yeast

Mutation

Promoter

Gal1pr

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-fSNAPc

Plasmid

#106986

Purpose

open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

fSNAP

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-TO-Casp-rastvTEV-teTVMV0

Plasmid

#116096

Purpose

Expresses a combination of proteases and their reporter

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Casp-rastvTEV-teTVMV0

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a_ TET1 CXXC only

Plasmid

#124084

Purpose

Expression of CXXC domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 - CXXC domain (TET1 Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

EF1 alpha

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBS537 tet-hCMV-GFPcre

Plasmid

#11960

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2006

Availability

Academic Institutions and Nonprofits only
pTet-O-APOE-E4-T2A-Puro

Plasmid

#190813

Purpose

Express APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance gene

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Tags

Expression

Mutation

E4

Promoter

Tet-O

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTet-O-APOE-E3-T2A-Puro

Plasmid

#190812

Purpose

Express APOE E3 allele from tet-inducible promoter, co-expressing Puro resistance gene

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Tags

Expression

Mutation

E3

Promoter

Tet-O

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTet-O-APOE-E2-T2A-Puro

Plasmid

#190811

Purpose

Express APOE E2 allele from tet-inducible promoter, co-expressing Puro resistance gene

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Tags

Expression

Mutation

E2

Promoter

Tet-O

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAB303-Tier1-PhCMV-TetR_VPR

Plasmid

#169575

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline-dependent VPR-based transactiavtor (PhCMV-TetR-VPR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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