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M23: pHR-SFFVp-FUSN::mCherry::SspB

Plasmid

#122148

Purpose

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Publication

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Tol2-elavl3-H2B-GCaMP6s

Plasmid

#59530

Purpose

Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Publication

Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
H2B
GCaMP6s

Use

Transposable element

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pDEST/hCx43-EGFP-N1

Plasmid

#40907

Depositor

Robin Shaw

Publication

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 (GJA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223-MET

Plasmid

#23889

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MET (MET Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLX307 Luciferase

Plasmid

#117734

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Publication

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pTDH3-dCas9

Plasmid

#46920

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9

Use

CRISPR

Expression

Yeast

Promoter

TDH3

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pDONR223-ALK

Plasmid

#23917

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ALK (ALK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Publication

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLenti6/V5-p53_R280K

Plasmid

#22933

Depositor

Bernard Futscher

Publication

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

R280K mutant

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Flag-HA-USP16

Plasmid

#22595

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP16 (USP16 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Flag-HA-USP42

Plasmid

#22603

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP42 (USP42 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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hIRE1a wt

Plasmid

#20744

Depositor

Fumihiko Urano

Publication

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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His6-TEV-CRBN-midi

Plasmid

#215330

Purpose

CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

Depositor

Alessio Ciulli

Publication

Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

Insert

CRBN (CRBN Human)

Tags

6xHis and TEV

Expression

Bacterial

Mutation

contains amino acids 41-187 followed by a Gly-Ser…

Promoter

lacI

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mCherry-GAL9

Plasmid

#166689

Purpose

For creating a stable mCherry-GAL9 cell line

Depositor

Alan Sabirsh

Publication

Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

Insert

LGALS9 (LGALS9 Human)

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pX330a dCas9-LSD1

Plasmid

#92362

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-LSD1

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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phDLX1-N174

Plasmid

#60859

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

Depositor

Andrew Yoo

Publication

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX1 (DLX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLKO.DEST.EGFP

Plasmid

#32684

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Publication

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDONR223-NTRK2

Plasmid

#23883

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NTRK2 (NTRK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Flag-HA-USP37

Plasmid

#22602

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP37 (USP37 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#190033

Purpose

vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

Depositor

Huawei Tong

Publication

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized hfCas13X

Expression

Mammalian

Mutation

Y672A,Y676A

Promoter

CBh, SV40, hU6

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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