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pME-CreLite

Plasmid

#131782

Purpose

Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

Depositor

George Eisenhoffer

Publication

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

Cre/Lox and Gateway Cloning

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-VAMP2:HRP

Plasmid

#60659

Purpose

Labels synaptic vesicles for electron microscopy

Depositor

Scott Sternson

Publication

Atasoy et al Nat Neurosci. 2014 Dec;17(12):1830-9. do

Insert

VAMP2:HRP

Use

AAV and Cre/Lox

Tags

Myc

Promoter

CAG

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
V621 pGEX-4T-LP - Acceptor

Plasmid

#11715

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

GST

Expression

Bacterial

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+PhyBΔCreC

Plasmid

#131781

Purpose

Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.

Depositor

George Eisenhoffer

Publication

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PhyBCreC

Use

Cre/Lox; Zebrafish expression

Expression

Mammalian

Promoter

CMV

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pOTF3-AK (clone3)

Plasmid

#21165

Depositor

James Thomson

Publication

Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Insert

POUF5F1 (genomic DNA) (POU5F1 Human)

Use

Cre/Lox; Homologous recombination

Mutation

NeoR, EGFP, and SV40 polyA are between the two ge…

Available since

June 25, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-TRE-DIO-hM4Di-mCherry

Plasmid

#182532

Purpose

This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.

Depositor

William Wisden

Publication

Yu et al Science. 2022 Jul;377(6601):63-72. doi:

Insert

AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

hM4Di-mCherry

Expression

Mammalian

Promoter

tetO promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-LACTB-dAPEX2

Plasmid

#117180

Purpose

Plasmid name in publication: pAAV-DIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Publication

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

LACTB-dAPEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA

Plasmid

#37087

Purpose

Expresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibition

Depositor

Bernardo Sabatini

Publication

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

NpHR3.0-EYFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV.GFAP.iGluSnFR3.v857.PDGFR

Plasmid

#178336

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Publication

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV GFAP iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-ArchT-GFP

Plasmid

#58851

Purpose

AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner

Depositor

Edward Boyden

Publication

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHD066

Plasmid

#84032

Purpose

Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafish

Depositor

Daniel Hesselson, Didier Stainier

Publication

Hesselson et al Curr Biol. 2011 Apr 26;21(8):712-7. doi:

Inserts

Cre
Venus

Use

Cre/Lox; Zebrafish transgenesis

Tags

mCherry

Promoter

Crystalin and Elastase

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Col1A2:TFPnls-T2a-mErt-Cre-Ert

Plasmid

#111151

Purpose

Fibroblasts specific inducible Cre

Depositor

Elly Tanaka

Insert

TFPnls-T2a-mErt-Cre-Ert

Use

Cre/Lox

Mutation

wt

Promoter

Col1A2

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-synP-FLEX-splitTVA-EGFP

Plasmid

#52474

Purpose

Expresses splitTVA-EGFP

Depositor

Ian Wickersham

Publication

Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

Insert

splitTVA-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Synapsin-1

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Puro.let-7gsm.Cre

Plasmid

#17410

Depositor

Tyler Jacks

Publication

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

Let-7g mutant

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

mutated version of let-7g

Available since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCre-P2A-mCherry

Plasmid

#212105

Purpose

Expression of Cre and mCherry in mammalian cells

Depositor

Daekee Lee

Insert

Cre recombinase and mCherry

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pJW1583

Plasmid

#121054

Purpose

GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC^AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, C. elegans codon optimized GFP, and auxin…

Expression

Worm

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-CMV-IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

Plasmid

#171619

Purpose

AAV floxed Soma-targeted Cal-Light with KA2

Depositor

Hyungbae Kwon

Publication

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-pAceR-Kv2.1PR

Plasmid

#195534

Purpose

Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Publication

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pHA1

Plasmid

#85709

Purpose

This plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

Depositor

Ulrich Koszinowski, Martin Messerle

Publication

Adler et al J Virol. 2000 Aug;74(15):6964-74.

Type

Empty backbone

Use

Cre/Lox; Herpesvirus cloning

Expression

Bacterial

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon DREADD Gi-mCherry

Plasmid

#177669

Purpose

Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

Depositor

Karl Deisseroth

Publication

Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

Insert

DREADD Gi-mCherry

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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