We narrowed to 23,974 results for: nes

1. pAGM16429

   Plasmid

   #105405

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   6xHis-NES(PKI)-GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI/ BpiI restriction sites removed

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGM16456

   Plasmid

   #105402

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   6xHis-NES(Rev)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI/ BpiI restriction sites removed

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AgDD

   Plasmid

   #78289

   Purpose

   create protein aggregates in living cells

   Depositor

   Thomas Wandless

   Article

   Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

   Insert

   NES-L106P-sfGFP (FKBP1A Human)
   

   Use

   Tags

   MLALKLAGLDI

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDV24_AAV2_DART-VADAR_iRFP720-sensor

   Plasmid

   #200411

   Purpose

   AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   DART VADAR sensor
   

   Use

   AAV and Synthetic Biology

   Tags

   MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PM-APEX2

   Plasmid

   #215557

   Purpose

   Stable, inducible expression in plasma membrane

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   Lyn11-EGFP-Flag tag-APEX2-NES
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJJiaDEST-APEX2

   Plasmid

   #149517

   Purpose

   N-termial APEX2 tag. Gateway destination vector for mammanlian expression

   Depositor

   Vojo Deretic

   Article

   Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

   Type

   Empty backbone

   Use

   Tags

   FLAG-APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDN80

   Plasmid

   #61349

   Purpose

   LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   AsLOV2 wt and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SLF060: Lenti-CaMKIIa-jRGECO1a

   Plasmid

   #112282

   Purpose

   For lentiviral or direct expression of cytosolic jRGECO1a in excitatory neurons

   Depositor

   Adam Cohen

   Article

   Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

   Insert

   jRGECO1a
   

   Use

   Lentiviral

   Tags

   NES-6xHIS

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIa

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)

   Plasmid

   #164184

   Purpose

   Freeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)

   Depositor

   Thomas Oertner

   Article

   Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

   Inserts

   CamPARI2
   Human α Tubulin
   

   Use

   AAV

   Tags

   6 His tag and NES

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGP-CMV-NEW-fRGECO2

    Plasmid

    #125248

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MTK4a_002

    Plasmid

    #123816

    Purpose

    Encodes Nuclear Export sequence as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MTK3a_002

    Plasmid

    #123755

    Purpose

    Encodes Nuclear Export sequence as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQP-2376

    Plasmid

    #138974

    Purpose

    Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-PKAsub-YPet-Kras

    Plasmid

    #138206

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub-YPet-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NLS-AgDD

    Plasmid

    #80625

    Purpose

    create nuclear protein aggregates in mammalian cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NLS-NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MPPKKKRKVLALKLAGLDI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEarleyGate 100RMOA mPing

    Plasmid

    #196137

    Purpose

    Measures mPing transposition in plants

    Depositor

    Charles Nathan Hancock

    Article

    Renken et al MicroPubl Biol. 2023 May 16;2023:10.1791

    Inserts

    Pong TPase LA T2A ORF1SC1 ONE
    mPing
    

    Use

    Binary

    Tags

    Expression

    Plant

    Mutation

    TPase NES sequence mutated L418A, L420A; T2A pept…

    Promoter

    35S and RPS5a

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

    Plasmid

    #164193

    Purpose

    Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

    Inserts

    CamPARI2
    Human α Tubulin
    

    Use

    AAV

    Tags

    6 His tag and NES

    Expression

    Mammalian

    Mutation

    F391W-L398V

    Promoter

    Syn

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Pvha6-AgDD

    Plasmid

    #80626

    Purpose

    create protein aggregates in C. elegans

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MLALKLAGLDI

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Bxb1_jRCaMP1b

    Plasmid

    #183758

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    jRCaMP1b
    

    Use

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-AMPKsub-YPet-Kras

    Plasmid

    #84638

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-Kras
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge