165,775 results

1. pT7-VP2SA11

   Plasmid

   #89163

   Purpose

   Express Rotavirus SA11 VP2 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP2
   

   Expression

   Mammalian

   Promoter

   T7 promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM468-EGFP

   Plasmid

   #108434

   Purpose

   ORBIT integrating plasmid for C-terminal tagging with EGFP.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   attB-eGFP-4xGly-TEV-Flag-6xHis
   

   Use

   Orbit integrating plasmid

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

   Plasmid

   #165450

   Purpose

   Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9N-IntN
   3x Opn1mw promoter-targeting sgRNAs
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6 and short human rhodopsin promoter

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-PLXNB2

   Plasmid

   #86237

   Purpose

   Lentivirus for expression of human Plexin-B2

   Depositor

   Roland Friedel

   Article

   Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

   Insert

   Plexin-B2 (PLXNB2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   full length PLXNB2, CRISPR resistant

   Promoter

   human PGK promoter

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IZ-HaloTag7-CHMP4A

   Plasmid

   #184895

   Purpose

   Stable expression of HaloTag7-CHMP4A in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

   Insert

   CHMP4A (CHMP4A Human)
   

   Tags

   HaloTag7

   Expression

   Mammalian

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Linear-GFP

   Plasmid

   #226258

   Purpose

   Production of linear RNA via IVT

   Depositor

   Prashant Mali

   Article

   Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

   Insert

   EGFP
   

   Use

   In vitro transcription

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.2/EV

   Plasmid

   #120833

   Purpose

   Control vector, 20nt random sequence between gateway recombination sites

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-SauriCas9

   Plasmid

   #135964

   Purpose

   Expresses SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-Neo HA-hSnoN

   Plasmid

   #10908

   Depositor

   Bob Weinberg

   Article

   Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

   Insert

   SnoN (SKIL Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

10. 457 p3E-2A-EGFPpA

    Plasmid

    #195967

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-EGFP
    

    Use

    Other

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. epi-BE4max

    Plasmid

    #135975

    Purpose

    Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST17-UbE2R2

    Plasmid

    #15806

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid

    #227327

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCherry-NLS

    Plasmid

    #39319

    Depositor

    Martin Offterdinger

    Article

    Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

    Insert

    mCherry-3xNLS
    

    Tags

    3xSV40LT NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

    Plasmid

    #230075

    Purpose

    Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

    Depositor

    Sumeet Pal Singh

    Article

    Hovhannisyan et al bioRxiv 2025.05.23.655316

    Insert

    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
    

    Use

    Cre/Lox; Zebrafish expression

    Promoter

    fabp10a

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-F-AF9

    Plasmid

    #49428

    Purpose

    Mammalian expression of Flag-tagged human AF9

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    AF-9 (MLLT3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPGK-T7/2-CD44v2-10 (fl)

    Plasmid

    #137824

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v2-10 (fl) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and mutations A282T, T483A, N535D, S5…

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDV002

    Plasmid

    #191823

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST40-Arl13b11

    Plasmid

    #40873

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    no stop codon

    Promoter

    CMV

    Available Since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

20. FokI-dCas9

    Plasmid

    #52970

    Purpose

    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

    Depositor

    David Liu

    Article

    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Fok1 fused to dCas9
    

    Use

    CRISPR

    Tags

    Fok1 nuclease (homodimeric wildtype) and NLS

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    CMV

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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