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Dendra2-Vinculin-N-21

Plasmid

#57749

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

NM_003373.3 (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Ad-EFNA1

Plasmid

#102724

Purpose

Expression of EphrinA1

Depositor

Jin Chen

Article

Brantley-Sieders et al Cancer Res. 2006 Nov 1;66(21):10315-24.

Insert

EFNA1 (EFNA1 Human)

Use

Adenoviral

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRSpuro shID1#6

Plasmid

#19165

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 1 (ID1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pWP1_mHAL

Plasmid

#102318

Purpose

expression of mouse cDNA of HAL refractory to the guide in plasmid 4

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Hal (Hal Mouse)

Use

Lentiviral

Promoter

EF1alpha

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔRING-Box

Plasmid

#105520

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFIV-H1-Puro-hPolß

Plasmid

#18663

Depositor

Robert Sobol

Article

Trivedi et al Molecular Pharmacology 2008;74(2):505-16

Insert

shRNA specific to human DNA polymerase beta (POLB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

none

Available Since

Oct. 24, 2008

Availability

Academic Institutions and Nonprofits only
attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-puro/HA-FLAG-RBP2 H483A

Plasmid

#14803

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

changed His 483 to Ala

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV_Flex_syn_SpikeyGi2_kv2.1

Plasmid

#195314

Purpose

Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSyn

Depositor

Vincent Pieribone

Article

Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

Insert

SpikeyGi2

Use

AAV

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG

Plasmid

#132683

Purpose

AAV plasmid encoding mouse CNDP1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Carnosine dipeptidase 1 (Cndp1 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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CMV:eGFP-p2A-HA-GPR151

Plasmid

#190133

Purpose

expresses eGFP-p2A-HA-GPR151 (wild-type variant) in mammalian cells

Depositor

Joshua Knowles

Article

Bielczyk-Maczynska et al Nat Commun. 2022 Dec 1;13(1):7408. doi:

Insert

eGFP-p2A-HA-GPR151

Use

Lentiviral

Tags

HA, eGFP, and p2A

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-mTDGa.0

Plasmid

#81050

Purpose

bacterial expression of murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pEDF-PhdRS

Plasmid

#127445

Purpose

Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

Depositor

Wenshe Liu

Article

Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Mutation

N346A-C348A mutations on PylRS

Promoter

araBAD

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCXLE-hS + eGFP

Plasmid

#74945

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

Sox2 (SOX2 Human)

Expression

Mammalian

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only
Human HMG-CoA lyase (HL)

Plasmid

#52433

Purpose

bacterial expression of human HMG-CoA lyase (HL)

Depositor

Henry Miziorko

Article

Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

Insert

HMG CoA lyase (HMGCL Human)

Tags

His and S

Expression

Bacterial

Promoter

T7

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
pMIR-CDH1 wtUTR

Plasmid

#25038

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 3'UTR (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
plenti hSyn PGK1-HALO

Plasmid

#220910

Purpose

Neuronal specific expression of the fusion construct PGK1-HALO

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-HALO (PGK1 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

hSyn

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRSpuro shID3#1

Plasmid

#19163

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
pAS4.1w-tet-on-NORAD

Plasmid

#125010

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

Homo sapiens non-coding RNA activated by DNA damage (NORAD), long non-coding RNA (NORAD Human)

Expression

Mammalian

Promoter

TRE-Tight

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b-eGO-Matryoshka-LE-LP

Plasmid

#100017

Purpose

Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

cpEGFP-LSSmOrange

Tags

6x HIS tag

Expression

Bacterial

Mutation

Leu and Glu N-term linker and Leu and Pro C-term …

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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