164,852 results

1. TFORF0098

   Plasmid

   #142460

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MSGN1 (MSGN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0041-RanCas13b crRNA backbone

   Plasmid

   #103852

   Purpose

   For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-RanCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6-GFP

   Plasmid

   #35637

   Depositor

   Daniel Haber

   Article

   Singh et al Cell. 2012 Feb 17;148(4):639-50.

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. TFORF3114

   Plasmid

   #144590

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   BHLHA15 (BHLHA15 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166015

   Purpose

   mammalian expression of HCoV-OC43 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   HCoV-OC43 S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPROBE-gfp[tagless]

   Plasmid

   #40164

   Depositor

   Steven Lindow

   Article

   Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   Promotor probe
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEvol-pAzFRS.1.t1

   Plasmid

   #73547

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAzFRS.1.t1
   pAzFRS.1.t1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. hTLR4

   Plasmid

   #13086

   Depositor

   Ruslan Medzhitov

   Insert

   TLR4 (TLR4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PRKAG3 gRNA (BRDN0001145578)

   Plasmid

   #77295

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKAG3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKAG3 (PRKAG3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PRKAG3 gRNA (BRDN0001147132)

    Plasmid

    #77296

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PRKAG3 gRNA (BRDN0001146704)

    Plasmid

    #77294

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX458-HypaCas9

    Plasmid

    #190604

    Purpose

    PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF2474

    Plasmid

    #144029

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ONECUT3 (ONECUT3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Alpha1

    Plasmid

    #118044

    Purpose

    GoldenBraid2.0 Alpha 1 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pscAAV-CBh-tagBFP2-WPRE3-enSV40pA

    Plasmid

    #194246

    Purpose

    Expresses tagBFP2

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    tagBFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CBh

    Available Since

    May 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pMXs-DDX3X

    Plasmid

    #70648

    Purpose

    Expresses WT DDX3X in mammalian cells under blasticidin selection.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Retroviral

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

17. GAL4-DrBphP

    Plasmid

    #158986

    Purpose

    Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cells

    Depositor

    Liangcai Gu

    Article

    Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

    Insert

    GAL4-DrBphP
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TurboID-EV

    Plasmid

    #215085

    Purpose

    Expresses TurboID in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-sfGFP[N150TAA]

    Plasmid

    #164581

    Purpose

    superfolder GFP with N150TAA mutation

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-150TAA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N150TAA Mutation

    Promoter

    T7

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. NbV5-GFP2

    Plasmid

    #201477

    Purpose

    BRET2 detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-GFP2
    

    Use

    Tags

    GFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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