164,853 results

1. pMTS-hFluc

   Plasmid

   #177716

   Purpose

   Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFluc
   

   Use

   Tags

   Mitochondrial Targeting Sequence MTS SS

   Expression

   Bacterial, Mammalian, and Y…

   Mutation

   Promoter

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ai62(TITL-tdT) Flp-in replacement vector

   Plasmid

   #61576

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   chromatin insulator flanked TRE-LSL-tdTomato
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDB1 (CK2alpha)

   Plasmid

   #27083

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha (CSNK2A1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR L3-4XmCherry-HA-L2

   Plasmid

   #32315

   Depositor

   Steve Stowers

   Article

   Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

   Insert

   4XmCherry-HA
   

   Use

   Gateway multisite entry vector

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. Gs(short)-CASE

   Plasmid

   #168124

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG1

   Expression

   Mammalian

   Mutation

   NLuc is inserted at N136/V137 within GNAS

   Promoter

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-FGFR2

   Plasmid

   #116741

   Purpose

   Lentiviral expression of FGFR2

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   FGFR2 (FGFR2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. plRL118

   Plasmid

   #163632

   Purpose

   Renilla luciferase reporter plasmid; Giles integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Mycobacterial Optimized Promoter (MOP)

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-ERBB2-V5/HIS

   Plasmid

   #201103

   Purpose

   expression of human ERBB2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human ERBB2 receptor tyrosine kinase, full length, wildtype (ERBB2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-c-myc-zeo

   Plasmid

   #17758

   Depositor

   Susan Lindquist

   Article

   Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

   Insert

   c-myc (MYC Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Rc/CMV cyclin A

    Plasmid

    #8959

    Depositor

    Bob Weinberg

    Article

    Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

    Insert

    cyclin A (CCNA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. mc2 mCherry

    Plasmid

    #206219

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 AIF 1-90 mCherry FRB

    Plasmid

    #67530

    Purpose

    For expression of mCherry fused FRB to the mitochondrial innermembrane

    Depositor

    Stephen Tait

    Article

    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

    Inserts

    AIF a.a. 1 - 90
    mCherry
    FRB
    

    Use

    Tags

    AIF 1 - 90, FRB, and mCherry

    Expression

    Mammalian

    Mutation

    T->L

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF1918

    Plasmid

    #142886

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RUNX3 (RUNX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWPXL-c-Myc

    Plasmid

    #36980

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

    Plasmid

    #160369

    Purpose

    Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    GPR143 (GPR143 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet-3xHA

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57-Simple-gRNA backbone

    Plasmid

    #51306

    Purpose

    gRNA expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Type

    Empty backbone

    Use

    CRISPR; Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-C-Solo-mStayGold-TOMM20

    Plasmid

    #227307

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mc2 neo

    Plasmid

    #206214

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 5 pCOLI_A_STREP

    Plasmid

    #160147

    Purpose

    N-term 2xStrepII 3C cleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. eGFP-ova-C1

    Plasmid

    #163524

    Purpose

    Expresses both eGFP and chicken ovalbumin in mammalian cells

    Depositor

    Thomas Weber

    Insert

    enhanced-GFP-chicken ovalbumin tandem
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge